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No evidence of enteroviruses in the intestine of patients with type 1 diabetes
- Source :
- Europe PubMed Central, Diabetologia; Vol 55
- Publication Year :
- 2012
-
Abstract
- AIMS/HYPOTHESIS: The purpose of this study was to investigate whether the gut mucosa is a reservoir for enterovirus persistence in patients with type 1 diabetes.METHODS: Small intestine biopsy samples from 25 individuals at different stages of type 1 diabetes, 21 control individuals and 27 individuals with coeliac disease were analysed for the presence of enterovirus RNA by using both radioactive in-situ hybridisation and real-time RT-PCR and for the presence of enterovirus proteins by immunostaining with antibodies against VP1 and VP4-2-3 capsid proteins and virus polymerase. Lymphocytic enteropathy and serum anti-VP1 antibodies were also evaluated at the time of biopsy. Moreover, high-throughput sequencing was performed to identify viral transcripts or genomes.RESULTS: Enterovirus was not detected by in-situ hybridisation or RT-PCR in any of the individuals tested. Immunohistology revealed a few stained cells in the intestinal epithelium in a low number of individuals, with no difference between diabetic and non-diabetic individuals. Levels of serum IgG against VP1 did not differ between control individuals and those with diabetes or coeliac disease and no evidence of diabetes-related lymphocytic enteropathy was detected. High-throughput sequencing did not reveal specific enterovirus sequences in the gut mucosa of individuals with type 1 diabetes.CONCLUSIONS/INTERPRETATION: Prolonged/persistent enterovirus infections in gut mucosa are not common in patients with type 1 diabetes
- Subjects :
- Adult
Male
Adolescent
viruses
Endocrinology, Diabetes and Metabolism
030209 endocrinology & metabolism
medicine.disease_cause
Virus Replication
Coeliac disease
Virus
03 medical and health sciences
Young Adult
0302 clinical medicine
Diabetes mellitus
Biopsy
Internal Medicine
medicine
Enterovirus Infections
Humans
Intestinal Mucosa
Child
In Situ Hybridization
030304 developmental biology
Aged
Enterovirus
0303 health sciences
Type 1 diabetes
biology
medicine.diagnostic_test
business.industry
Reverse Transcriptase Polymerase Chain Reaction
virus diseases
Middle Aged
medicine.disease
Virology
Immunohistochemistry
3. Good health
Diabetes Mellitus, Type 1
Child, Preschool
biology.protein
RNA, Viral
Female
Antibody
business
Immunostaining
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Europe PubMed Central, Diabetologia; Vol 55
- Accession number :
- edsair.doi.dedup.....6fee186151c66e131688bfe7b5ebe18c