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Vascular endothelial growth factor production in polymyalgia rheumatica
- Publication Year :
- 2000
-
Abstract
- Objective. To evaluate peripheral production and synovial expression of vascular endothelial growth factor (VEGF) in polymyalgia rheumatica (PMR). Methods. Circulating levels of VEGF in PMR (serum concentration and in vitro release by peripheral blood mononuclear cells [PBMC]) were investigated by enzyme-linked immunosorbent assay. Local expression of VEGF in shoulder synovial tissue was investigated by immunohistochemical analysis. Investigations were performed in patients with active, untreated disease and in patients treated with corticosteroids. Results. VEGF serum concentrations were significantly higher in untreated PMR patients than in normal control subjects. During steroid treatment, VEGF serum concentrations reached their lowest level after the sixth month of treatment. PBMC isolated from untreated PMR patients spontaneously secreted a higher amount of VEGF compared with PBMC from control subjects. Corticosteroid therapy did not affect the ability of PBMC to produce VEGF. Immunohistochemical staining performed on shoulder synovial tissue showed VEGF expression in both the lining layer and the sublining area. In 3 of 4 treated patients, no VEGF staining was found in synovial tissue during corticosteroid therapy. VEGF expression correlated with vessel density, but was not associated with avb3 and avb5 integrin expression. Conclusion. Peripheral and local VEGF releases have different responses to steroid treatment in PMR. The lack of response to corticosteroids by peripheral VEGF production supports the hypothesis that systemic involvement is dominant in PMR. At the synovial level, VEGF production is linked to vascular proliferation and is thus directly involved in the pathogenesis of synovitis.
- Subjects :
- musculoskeletal diseases
Vascular Endothelial Growth Factor A
Pathology
medicine.medical_specialty
Integrins
medicine.medical_treatment
Immunology
Endothelial Growth Factors
Peripheral blood mononuclear cell
Polymyalgia rheumatica
chemistry.chemical_compound
Rheumatology
Synovitis
Immunology and Allergy
Medicine
Protein Isoforms
Pharmacology (medical)
Lymphokines
business.industry
Vascular Endothelial Growth Factors
Growth factor
Synovial Membrane
medicine.disease
Immunohistochemistry
Vascular endothelial growth factor
Vascular endothelial growth factor A
medicine.anatomical_structure
chemistry
Polymyalgia Rheumatica
Leukocytes, Mononuclear
Synovial membrane
business
Vascular endothelial growth factor production
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....6fe30bf5ffc92e666f234fb890cc62d3