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A paperless autoimmunity laboratory: myth or reality?
- Source :
- Annales de biologie clinique. 74(4)
- Publication Year :
- 2016
-
Abstract
- Testing for antinuclear antibodies is the most frequently prescribed analysis for the diagnosis of rheumatic diseases. Indirect immunofluorescence remains the gold standard method for their detection despite the increasing use of alternative techniques. In order to standardize the manual microscopy reading, automated acquisition and interpretation systems have emerged. This publication enables us to present our method of interpretation and characterization of antinuclear antibodies based on a cascade of analyses and to share our everyday experience of the G Sight from Menarini. The positive/negative discrimination on Hep cells 2000 is correct in 85% of the cases. In most of the false negative results, it is a question of aspecific or low titers patterns, but a few cases of SSA speckled patterns of low titers demonstrated a probability index below 8. Regarding the pattern recognition, some types and mixed patterns are not properly recognized. Concerning the probability index correlated in some studies to final titer, the weak fluorescence of certain patterns and the random presence of artifacts that distort the index don't lead us to continue it in our daily practice. In conclusion, automated reading systems facilitate the reporting of results and traceability of patterns but still require the expertise of a laboratory technologist for positive/negative discrimination and for pattern recognition.
- Subjects :
- Paper
medicine.medical_specialty
Anti-nuclear antibody
media_common.quotation_subject
Autoimmunity
Laboratory Technologist
030204 cardiovascular system & hematology
Sensitivity and Specificity
03 medical and health sciences
0302 clinical medicine
Reading (process)
Daily practice
medicine
Image Processing, Computer-Assisted
Humans
Medical physics
Cells, Cultured
media_common
030203 arthritis & rheumatology
Automation, Laboratory
Indirect immunofluorescence
business.industry
General Medicine
Gold standard (test)
Reference Standards
Titer
Antibodies, Antinuclear
Pattern recognition (psychology)
Critical Pathways
business
Laboratories
Subjects
Details
- ISSN :
- 19506112
- Volume :
- 74
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Annales de biologie clinique
- Accession number :
- edsair.doi.dedup.....6fd5caebf044cb22184b1e0babe0e363