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Monoclonal Antibodies to Porcine Tumor Necrosis Factor Alpha: Development of an Enzyme-Linked Immunosorbent Assay
- Source :
- Journal of Immunoassay. 13:253-272
- Publication Year :
- 1992
- Publisher :
- Informa UK Limited, 1992.
-
Abstract
- Five hybridomas (4F4, 14H1, 9B4, 6E10, and 8G7) secreting antibodies to porcine tumor necrosis factor alpha (PTNF-alpha) were obtained from one fusion. Four of the 5 monoclonal antibodies (Mab) recognized recombinant PTNF-alpha (rPTNF-alpha) on western blot and were able to neutralize both rPTNF-alpha and native (released by porcine macrophages) PTNF (nPTNF-alpha, only 4F4, 14H1, and 9B4 tested for the neutralization of nPTNF-alpha) in vitro. A sandwich enzyme-linked immunosorbent assay (ELISA) for PTNF-alpha was developed using Mab 4F4 and purified rabbit polyclonal antibodies against PTNF-alpha. The test detected PTNF-alpha concentrations as low as 400 pg/ml and did not cross react with native porcine TNF-beta, recombinant human TNF-alpha, recombinant mouse TNF-beta or native mouse TNF-alpha. The Mabs and the ELISA should be useful for assessing PTNF-alpha levels in swine serum during disease processes and possibly for alleviation of toxic effects of TNF-alpha.
- Subjects :
- Swine
medicine.drug_class
medicine.medical_treatment
Immunology
Enzyme-Linked Immunosorbent Assay
Biology
Monoclonal antibody
Sensitivity and Specificity
Neutralization
law.invention
Western blot
Neutralization Tests
law
medicine
Animals
Pharmacology
Hybridomas
medicine.diagnostic_test
Tumor Necrosis Factor-alpha
Antibodies, Monoclonal
Molecular biology
Recombinant Proteins
In vitro
Cytokine
Evaluation Studies as Topic
biology.protein
Recombinant DNA
Tumor necrosis factor alpha
Antibody
Subjects
Details
- ISSN :
- 01971522
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Journal of Immunoassay
- Accession number :
- edsair.doi.dedup.....6fcbed8dd718fb6b0fa76dcdbfd94989