Back to Search
Start Over
Compensation by reduced L-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor responses in a mouse model with reduced γ-aminobutyric acid type A receptor-mediated synaptic inhibition
- Source :
- Journal of Neuroscience Research. 85:668-672
- Publication Year :
- 2007
- Publisher :
- Wiley, 2007.
-
Abstract
- L-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor antagonists increase the threshold for electroshock-induced convulsions. Here, we show that a transgenic mouse line overexpressing cerebellum-restricted gamma-aminobutyric acid type A (GABA(A)) receptor alpha6 subunit in the hippocampal CA1 pyramidal cells (Thy1alpha6 mouse line) exhibits about a 20% increase in the electroshock current intensity inducing tonic hindlimb extension convulsion in 50% of the mice compared with that of their wild-type controls. AMPA receptor-mediated miniature excitatory postsynaptic currents (mEPSCs) in patch clamp recordings of CA1 pyramidal neurons in hippocampal slices had decreased amplitudes (8.4 +/- 2.2 pA) in the transgenics compared with the wild types (10.3 +/- 2.5 pA) but showed no change in current decay or frequency. Our results suggest that decreased AMPA-mediated neurotransmission might explain the increased threshold for electroconvulsions and warrant further studies on the regulation between various components of inhibition and excitation in neurons.
- Subjects :
- Male
Genetically modified mouse
medicine.medical_specialty
AMPA receptor
Hippocampal formation
Neurotransmission
Biology
Hippocampus
Aminobutyric acid
Mice
03 medical and health sciences
Cellular and Molecular Neuroscience
0302 clinical medicine
Seizures
Internal medicine
medicine
Animals
Receptors, AMPA
Patch clamp
030304 developmental biology
Electroshock
0303 health sciences
Pyramidal Cells
Glutamate receptor
Receptors, GABA-A
Electrophysiology
Mice, Inbred C57BL
Endocrinology
nervous system
Models, Animal
Synapses
Mice, Inbred CBA
Excitatory postsynaptic potential
Neuroscience
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 10974547 and 03604012
- Volume :
- 85
- Database :
- OpenAIRE
- Journal :
- Journal of Neuroscience Research
- Accession number :
- edsair.doi.dedup.....6fc90e2b75d24472b5c51ec6a83e4eb1