UPLC Orbitrap HRMS Analysis of Panax quinquefolium L. for Authentication of Panax Genus with Chemometric Methods

Ginsenosides in Panax quinquefolium L. were determined using developed ultra-performance liquid chromatography coupled to high resolution mass spectrometry (UPLC-HRMS) method with electrospray ionization and orbitrap MS analyzer in negative ionization mode. Optimal UPLC separation was achieved using a mixture of acetonitrile and water with 0.1% formic acid as the mobile phase in linear gradient elution. The MS parameters were optimized for reliable detection with enhanced selectivity and sensitivity, and improved identification and quantification of ginsenosides. The applicability of this method was demonstrated on ginsenosides from Panax quinquefolium L. (American ginseng), Panax ginseng (Chinese ginseng) and Panax notoginseng (Sanchi) roots and products. The differences between Chinese and Northern American Panax quinquefolium L., main roots and hair roots, and products from different pharmacy were investigated. The results were also confirmed by principal component analysis and partial least squares discriminatory analysis. It indicated that the strategy can be extended to rapid and accurate authentication of Panax genus.