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p53 and Cell Cycle Dependent Transcription of kinesin family member 23 (KIF23) Is Controlled Via a CHR Promoter Element Bound by DREAM and MMB Complexes
- Source :
- PLoS ONE, PLoS ONE, Vol 8, Iss 5, p e63187 (2013)
- Publication Year :
- 2013
- Publisher :
- Public Library of Science, 2013.
-
Abstract
- The microtubule-dependent molecular motor KIF23 (Kinesin family member 23) is one of two components of the centralspindlin complex assembled during late stages of mitosis. Formation of this complex is known as an essential step for cytokinesis. Here, we identified KIF23 as a new transcriptional target gene of the tumor suppressor protein p53. We showed that p53 reduces expression of KIF23 on the mRNA as well as the protein level in different cell types. Promoter reporter assays revealed that this repression results from downregulation of KIF23 promoter activity. CDK inhibitor p21(WAF1/CIP1) was shown to be necessary to mediate p53-dependent repression. Furthermore, we identified the highly conserved cell cycle genes homology region (CHR) in the KIF23 promoter to be strictly required for p53-dependent repression as well as for cell cycle-dependent expression of KIF23. Cell cycle- and p53-dependent regulation of KIF23 appeared to be controlled by differential binding of DREAM and MMB complexes to the CHR element. With this study, we describe a new mechanism for transcriptional regulation of KIF23. Considering the strongly supporting function of KIF23 in cytokinesis, its p53-dependent repression may contribute to the prevention of uncontrolled cell growth.
- Subjects :
- Transcription, Genetic
KIF23
Mice
Molecular cell biology
Basic Cancer Research
Protein Isoforms
Promoter Regions, Genetic
Multidisciplinary
Protein Stability
Cell Cycle
Kv Channel-Interacting Proteins
Cell cycle
Cell Cycle Gene
Oncology
Kinesin
Medicine
Microtubule-Associated Proteins
Cell Division
Research Article
Protein Binding
Cyclin-Dependent Kinase Inhibitor p21
Science
DNA transcription
Biology
Response Elements
Molecular Genetics
Genetics
Human Cloning
Animals
Humans
Gene Regulation
Gene Silencing
RNA, Messenger
Psychological repression
Mitosis
Cytokinesis
Centralspindlin complex
HCT116 Cells
Molecular biology
Oncogene Proteins v-myb
Repressor Proteins
Gene Expression Regulation
NIH 3T3 Cells
Gene expression
Tumor Suppressor Protein p53
Cloning
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....6fae3b49eeede887dbcd32bc7e8ab05d