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Immunohistochemical Localization of Histamine Receptors in Rat Cochlea
- Source :
- The Laryngoscope. 114:2249-2251
- Publication Year :
- 2004
- Publisher :
- Wiley, 2004.
-
Abstract
- Objective: Histamine may have physiologic functions in the inner ear. The locations of histamine receptors, however, have not yet been identified in the mammalian cochlea. The aim of this study was to investigate the localization of histamine receptor subtypes (H1, H2, and H3 receptors) in rat cochlea. Methods: Immunohistochemistry was performed with antibodies specific for each of the histamine receptors (H1, H2, and H3). To identify the type I and II spiral ganglion cells in the cochlea, some cryostat sections were double stained with antibodies to both a histamine receptor and neurofilament 200 kD, which predominantly stains type II spiral ganglion cells in the cochlea. Results: All H1, H2, and H3 receptor immunoreactive staining was limited to the spiral ganglion cells of the cochlea. Spiral ganglion cells with positive immunoreactivity to the neurofilament 200 kD antibody were stained only slightly by histamine H1, H2, and H3 receptor antibodies, indicating that histamine receptor immunoreactivity is specific to type I ganglion cells. Conclusions: These findings indicate that histamine receptors are present in the cochlea and support the hypothesis that histamine plays a physiologic role in the cochlea. Key Words: Histamine, histamine receptor, immunohistochemistry, cochlea. Laryngoscope, 114:–, 2004
- Subjects :
- Pathology
medicine.medical_specialty
Biology
Sensitivity and Specificity
chemistry.chemical_compound
Histamine receptor
otorhinolaryngologic diseases
medicine
Animals
Receptors, Histamine H3
Receptors, Histamine H2
Inner ear
Receptors, Histamine H1
Rats, Wistar
Receptor
Spiral ganglion
Cochlea
Microscopy, Confocal
Immunohistochemistry
Rats
medicine.anatomical_structure
Otorhinolaryngology
chemistry
Models, Animal
Receptors, Histamine
Female
sense organs
Histamine H3 receptor
Histamine
Subjects
Details
- ISSN :
- 15314995 and 0023852X
- Volume :
- 114
- Database :
- OpenAIRE
- Journal :
- The Laryngoscope
- Accession number :
- edsair.doi.dedup.....6f8f5158fe78cea9909eda4589d64ee1