Back to Search
Start Over
Antagonistic roles of ubiquitin ligase HEI10 and SUMO ligase RNF212 regulate meiotic recombination
- Source :
- Nature genetics, vol 46, iss 2, Nature genetics
- Publication Year :
- 2014
- Publisher :
- eScholarship, University of California, 2014.
-
Abstract
- Crossover recombination facilitates the accurate segregation of homologous chromosomes during meiosis. In mammals, poorly characterized regulatory processes ensure that every pair of chromosomes obtains at least one crossover, even though most recombination sites yield non-crossovers. Designation of crossovers involves selective localization of the SUMO ligase RNF212 to a minority of recombination sites, where it stabilizes pertinent factors such as MutSĪ³ (ref. 4). Here we show that the ubiquitin ligase HEI10 (also called CCNB1IP1) is essential for this crossover/non-crossover differentiation process. In HEI10-deficient mice, RNF212 localizes to most recombination sites, and dissociation of both RNF212 and MutSĪ³ from chromosomes is blocked. Consequently, recombination is impeded, and crossing over fails. In wild-type mice, HEI10 accumulates at designated crossover sites, suggesting that it also has a late role in implementing crossing over. As with RNF212, dosage sensitivity for HEI10 indicates that it is a limiting factor for crossing over. We suggest that SUMO and ubiquitin have antagonistic roles during meiotic recombination that are balanced to effect differential stabilization of recombination factors at crossover and non-crossover sites.
- Subjects :
- Electrophoresis
Male
Mitotic crossover
Indoles
Ubiquitin-Protein Ligases
SUMO-1 Protein
Cell Cycle Proteins
Inbred C57BL
Genetic recombination
Medical and Health Sciences
Statistics, Nonparametric
Article
Chromosomal crossover
Ligases
Mice
Meiosis
Genetic
Spermatocytes
Genetics
In Situ Nick-End Labeling
Crossing Over
Animals
Nonparametric
Crossing Over, Genetic
Polyacrylamide Gel
biology
Synaptonemal Complex
Ubiquitin
Statistics
Biological Sciences
Ubiquitin ligase
Mice, Inbred C57BL
Synaptonemal complex
biology.protein
Electrophoresis, Polyacrylamide Gel
Homologous recombination
Recombination
Developmental Biology
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Nature genetics, vol 46, iss 2, Nature genetics
- Accession number :
- edsair.doi.dedup.....6f706bde1a331a812d78696c219b2e9f