New method of screening and differentiation of exoenzymes from industrial strains

A technique to determine zymographic profiles suitable for different types of microorganims is described. This technique for analysing extracellular amylolytic and xylanolytic activity of the strains consists in the obtention of crude extracts from solidified media. Samples for enzymatic analysis were obtained by freezing (-20°C) a section of solified media and recovering the supernatant after centrifugation.