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A novel method for screening colorectal cancer by infrared spectroscopy of peripheral blood mononuclear cells and plasma
- Source :
- Journal of Gastroenterology. 51:214-221
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- Early detection of colorectal cancer (CRC) can reduce mortality and morbidity. Current screening methods include colonoscopy and stool tests, but a simple low-cost blood test would increase compliance. This preliminary study assessed the utility of analyzing the entire bio-molecular profile of peripheral blood mononuclear cells (PBMCs) and plasma using Fourier transform infrared (FTIR) spectroscopy for early detection of CRC. Blood samples were prospectively collected from 62 candidates for CRC screening/diagnostic colonoscopy or surgery for colonic neoplasia. PBMCs and plasma were separated by Ficoll gradient, dried on zinc selenide slides, and placed under a FTIR microscope. FTIR spectra were analyzed for biomarkers and classified by principal component and discriminant analyses. Findings were compared among diagnostic groups. Significant changes in multiple bands that can serve as CRC biomarkers were observed in PBMCs (p = ~0.01) and plasma (p = ~0.0001) spectra. There were minor but statistically significant differences in both blood components between healthy individuals and patients with benign polyps. Following multivariate analysis, the healthy individuals could be well distinguished from patients with CRC, and the patients with benign polyps were mostly distributed as a distinct subgroup within the overlap region. Leave-one-out cross-validation for evaluating method performance yielded an area under the receiver operating characteristics curve of 0.77, with sensitivity 81.5 % and specificity 71.4 %. Joint analysis of the biochemical profile of two blood components rather than a single biomarker is a promising strategy for early detection of CRC. Additional studies are required to validate our preliminary clinical results.
- Subjects :
- Adult
Male
0301 basic medicine
medicine.medical_specialty
Pathology
Colorectal cancer
Colonoscopy
Peripheral blood mononuclear cell
Gastroenterology
Young Adult
03 medical and health sciences
0302 clinical medicine
Internal medicine
Spectroscopy, Fourier Transform Infrared
Biomarkers, Tumor
medicine
Humans
Blood test
Prospective Studies
Early Detection of Cancer
Aged
Aged, 80 and over
Blood Specimen Collection
medicine.diagnostic_test
Receiver operating characteristic
business.industry
Middle Aged
Hepatology
medicine.disease
Colorectal surgery
030104 developmental biology
030220 oncology & carcinogenesis
Leukocytes, Mononuclear
Biomarker (medicine)
Female
Colorectal Neoplasms
business
Subjects
Details
- ISSN :
- 14355922 and 09441174
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- Journal of Gastroenterology
- Accession number :
- edsair.doi.dedup.....6f3af1e0cf0bf9c1be97251ae20caba9