Back to Search
Start Over
Insulin suppresses plasma concentration of vascular endothelial growth factor and matrix metalloproteinase-9
- Source :
- Diabetes care. 26(12)
- Publication Year :
- 2003
-
Abstract
- OBJECTIVE—We recently demonstrated a potent anti-inflammatory and thus a potential antiatherogenic effect of insulin in human aortic endothelial cells and mononuclear cells at physiologically relevant concentrations. We have now further investigated the anti-inflammatory suppressive action of insulin on vascular endothelial growth factor (VEGF) and matrix metalloproteinase (MMP)-9. VEGF and MMP-9 play a central regulatory role in angiogenesis, contribute to the pathogenesis of proliferative retinopathy, and have also been found to accelerate atherosclerosis. RESEARCH DESIGN AND METHODS—Insulin was infused (2 IU/h) in 5% dextrose (100 ml/h) and KCl (8 mmol/h) into 10 fasting, obese, nondiabetic subjects for 4 h. Subjects were also infused with 5% dextrose without insulin and with saline on two separate occasions. Blood samples were obtained at 0, 2, 4, and 6 h. RESULTS—Plasma insulin concentrations increased from a basal level of 12.5 ± 2.2 to 28.2 ± 3.3 μU/ml at 2 h and 24.4 ± 3.7 μU/ml at 4 h after insulin infusion. VEGF concentration decreased from 307.2 ± 163.8 pg/ml (100%) at 0 h to 73.5 ± 20.9% of the basal level at 2 h and 67.1 ± 23.2% at 4h. Plasma MMP-9 concentrations decreased from 375 ± 196.3 ng/ml (100%) at 0 h to 83 ± 22% of the basal level at 2 h and to 82 ± 21% of the basal level at 4 h (P < 0.05). Dextrose infusion alone did not change plasma VEGF concentration. However, plasma MMP-9 concentration increased significantly at 4 h following dextrose infusion alone (P < 0.05). Saline infusions without insulin caused no alteration in glucose, insulin, VEGF, or MMP-9. CONCLUSIONS—These observations may have implications for a potential antiretinopathic and antiatherosclerotic effect of insulin in the long term.
- Subjects :
- Adult
Vascular Endothelial Growth Factor A
medicine.medical_specialty
Angiogenesis
Endocrinology, Diabetes and Metabolism
medicine.medical_treatment
Matrix Metalloproteinase Inhibitors
Sodium Chloride
Peripheral blood mononuclear cell
chemistry.chemical_compound
Internal medicine
Diabetes mellitus
Blood plasma
Internal Medicine
medicine
Humans
Insulin
Obesity
Infusions, Intravenous
Saline
Advanced and Specialized Nursing
business.industry
Middle Aged
medicine.disease
Vascular endothelial growth factor
Endocrinology
Glucose
Basal (medicine)
chemistry
Matrix Metalloproteinase 9
business
Subjects
Details
- ISSN :
- 01495992
- Volume :
- 26
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Diabetes care
- Accession number :
- edsair.doi.dedup.....6f3a14139cd017675e738ccf0ff598dd