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Generation of induced pluripotent stem cell lines from Tibetan miniature pig
- Source :
- The Journal of biological chemistry. 284(26)
- Publication Year :
- 2009
-
Abstract
- Induced pluripotent stem cell (iPS) technology appears to be a general strategy to generate pluripotent stem cells from any given mammalian species. So far, iPS cells have been reported for mouse, human, rat, and monkey. These four species have also established embryonic stem cell (ESC) lines that serve as the gold standard for pluripotency comparisons. Attempts have been made to generate porcine ESC by various means without success. Here we report the successful generation of pluripotent stem cells from fibroblasts isolated from the Tibetan miniature pig using a modified iPS protocol. The resulting iPS cell lines more closely resemble human ESC than cells from other species, have normal karyotype, stain positive for alkaline phosphatase, express high levels of ESC-like markers (Nanog, Rex1, Lin28, and SSEA4), and can differentiate into teratomas composed of the three germ layers. Because porcine physiology closely resembles human, the iPS cells reported here provide an attractive model to study certain human diseases or assess therapeutic applications of iPS in a large animal model.
- Subjects :
- Homeobox protein NANOG
KOSR
Pluripotent Stem Cells
Swine
Rex1
Cellular differentiation
Cell Culture Techniques
Kruppel-Like Transcription Factors
Mice, Nude
Biology
LIN28
Kidney
Biochemistry
Proto-Oncogene Proteins c-myc
Kruppel-Like Factor 4
Mice
Molecular Basis of Cell and Developmental Biology
Animals
Humans
RNA, Messenger
Induced pluripotent stem cell
Molecular Biology
Reverse Transcriptase Polymerase Chain Reaction
Teratoma
Cell Differentiation
Cell Biology
Fibroblasts
Alkaline Phosphatase
Molecular biology
Embryonic stem cell
Microscopy, Fluorescence
Alkaline phosphatase
Swine, Miniature
Octamer Transcription Factor-3
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 284
- Issue :
- 26
- Database :
- OpenAIRE
- Journal :
- The Journal of biological chemistry
- Accession number :
- edsair.doi.dedup.....6f36e7bb148cffb560aebffa880d95bc