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Collagen type I enhances cell growth and insulin biosynthesis in rat pancreatic cells
- Source :
- Journal of Molecular Endocrinology. 67:135-148
- Publication Year :
- 2021
- Publisher :
- Bioscientifica, 2021.
-
Abstract
- Type I collagen (collagen I) is the most abundant component of the extracellular matrix (ECM) in the pancreas. We previously reported that collagen I-coated culture dishes enhanced proliferation of rat pancreatic β cell line, INS-1 cells, via up-regulation of β-catenin nuclear translocation. In this study, we further investigated the effects of collagen I on insulin production of INS-1 cells. The results indicate that insulin synthesis as well as cell proliferation is increased in the INS-1 cells cultured on the dishes coated with collagen I. Up-regulation of insulin-like growth factor 1 receptor (IGF-1R) on the INS-1 cells cultured on the collagen-coated dishes is involved in up-regulation of cell proliferation and increase of insulin biosynthesis; however, up-regulation of insulin secretion in the INS-1 cells on collagen I-coated dishes was further enhanced by inhibition of IGF-1R. Autophagy of INS-1 cells on collagen I-coated dishes was repressed via IGF-1R upregulation, and inhibition of autophagy with 3MA further enhanced cell proliferation and insulin biosynthesis but did not affect insulin secretion. E-cadherin/β-catenin adherent junction complexes are stabilized by autophagy. That is, autophagy negatively regulates the nuclear translocation of β-catenin that leads to insulin biosynthesis and cell proliferation. In conclusion, IGF-1R/downregulation of autophagy/nuclear translocation of β-catenin is involved in collagen I-induced INS-1 cell proliferation and insulin synthesis.
- Subjects :
- Cell Survival
medicine.medical_treatment
Gene Expression
Collagen Type I
Cell Line
Receptor, IGF Type 1
Extracellular matrix
Islets of Langerhans
Endocrinology
Downregulation and upregulation
Insulin Secretion
Autophagy
medicine
Animals
Insulin
Molecular Biology
beta Catenin
Cell Proliferation
Cell growth
Chemistry
Growth factor
Rats
Cell biology
Protein Transport
Cell culture
Biomarkers
Type I collagen
Protein Binding
Subjects
Details
- ISSN :
- 14796813 and 09525041
- Volume :
- 67
- Database :
- OpenAIRE
- Journal :
- Journal of Molecular Endocrinology
- Accession number :
- edsair.doi.dedup.....6f3652c28e5584994eb923eba8bd906b