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Anaerobic respiration coupled with mitochondrial fatty acid synthesis in wax ester fermentation by Euglena gracilis
- Source :
- Febs Letters
- Publication Year :
- 2018
- Publisher :
- John Wiley and Sons Inc., 2018.
-
Abstract
- In Euglena gracilis, wax ester fermentation produces ATP during anaerobiosis. Here, we report that anaerobic wax ester production is suppressed when the mitochondrial electron transport chain complex I is inhibited by rotenone, whereas it is increased by the uncoupler carbonyl cyanide m-chlorophenylhydrazone (CCCP). The ADP/ATP ratio in anaerobic cells is elevated by treatment with either rotenone or CCCP. Gene silencing experiments indicate that acyl-CoA dehydrogenase, electron transfer flavoprotein (ETF), and rhodoquinone (RQ) participate in wax ester production. These results suggest that fatty acids are synthesized in mitochondria by the reversal of β-oxidation, where trans-2-enoyl-CoA is reduced mainly by acyl-CoA dehydrogenase using the electrons provided by NADH via the electron transport chain complex I, RQ, and ETF, and that ATP production is highly supported by anaerobic respiration utilizing trans-2-enoyl-CoA as a terminal electron acceptor.
- Subjects :
- 0301 basic medicine
Anaerobic respiration
Euglena gracilis
030106 microbiology
ved/biology.organism_classification_rank.species
Cell Respiration
Biophysics
Dehydrogenase
wax ester fermentation
mitochondrial fatty acid synthesis
Mitochondrion
Biochemistry
Microbiology
Acyl-CoA Dehydrogenase
03 medical and health sciences
chemistry.chemical_compound
anaerobic respiration
Adenosine Triphosphate
Structural Biology
Rotenone
Genetics
Anaerobiosis
Molecular Biology
chemistry.chemical_classification
ved/biology
Uncoupling Agents
Fatty Acids
mitochondrial electron transfer system
Esters
Cell Biology
Electron acceptor
Electron transport chain
Mitochondria
Wax ester
Adenosine Diphosphate
Editor's Choice
030104 developmental biology
chemistry
Waxes
Fermentation
RNA Interference
Subjects
Details
- Language :
- English
- ISSN :
- 18733468 and 00145793
- Volume :
- 592
- Issue :
- 24
- Database :
- OpenAIRE
- Journal :
- Febs Letters
- Accession number :
- edsair.doi.dedup.....6f185fcae081afd1ddc4eedb35468326