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Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype
- Source :
- Prostaglandins & Other Lipid Mediators. 107:85-94
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- During the resolution of inflammation macrophages undergo functional changes upon exposure to pro-resolving agents in their microenvironment. Primarily, engulfment of apoptotic polymorphonuclear (PMN) cells promotes conversion of macrophages toward a pro-resolving phenotype characterized by reduced CD11b expression. These macrophages are not phagocytic, do not respond to TLR ligands, and express relatively high levels of the pro-resolving enzyme 12/15-lipoxygenase (LO). Here, we report that the immuno-regulatory lectin galectin-1 is selectively expressed by CD11bhigh, but not CD11blow macrophages. Upon exposure in vivo and ex vivo, galectin-1 directly promoted macrophage conversion from a CD11bhigh to a CD11blow phenotype and up-regulated the expression and activity of 12/15-LO. Moreover, galectin-1 treatment in vivo promoted the loss of phagocytic capacity (efferocytic satiation) in peritoneal macrophages and down-regulated secretion of TNF-α, IL-1β, and IL-10 upon LPS exposure. Our results suggest that galectin-1 could be an essential mediator in the control of macrophage function during the resolution of inflammation. Fil: Rostoker, Ran. University of Haifa. Faculty of Natural Sciences. Departament of Biology; Israel; Fil: Yaseen, Hiba. University of Haifa. Faculty of Natural Sciences. Departament of Biology; Israel; Fil: Schif Zuck, Sagie. University of Haifa. Faculty of Natural Sciences. Departament of Biology; Israel; Fil: Lichtenstein, Rachel G.. Ben-Gurion University. Department of Biotechnology Engineering; Israel; Fil: Rabinovich, Gabriel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Quimica Biologica; Argentina; Fil: Amiram, Ariel. University of Haifa. Faculty of Natural Sciences. Departament of Biology; Israel
- Subjects :
- Lipopolysaccharides
Male
EFFEROCYTOSIS
CIENCIAS MÉDICAS Y DE LA SALUD
Galectin 1
Physiology
Inmunología
Apoptosis
Inflammation
Biology
Arachidonate 12-Lipoxygenase
Biochemistry
Mice
In vivo
RESOLUTION OF INFLAMMATION
medicine
Animals
Arachidonate 15-Lipoxygenase
INFLAMMMATION
Macrophage
MACROPHAGES
Efferocytosis
Cells, Cultured
Galectin
Pharmacology
CD11 Antigens
Cell Biology
GALECTINS
Cell biology
Mice, Inbred C57BL
Medicina Básica
Phenotype
15-LIPOXYGENASE
RESOLUTION
Integrin alpha M
Enzyme Induction
Macrophages, Peritoneal
biology.protein
Cytokines
medicine.symptom
Ex vivo
Subjects
Details
- ISSN :
- 10988823
- Volume :
- 107
- Database :
- OpenAIRE
- Journal :
- Prostaglandins & Other Lipid Mediators
- Accession number :
- edsair.doi.dedup.....6f15e5fa6affded1f4f323af441cc326