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Modified Renshen Wumei Decoction Alleviates Intestinal Barrier Destruction in Rats with Diarrhea

Authors :
Yuanyuan He
Qiong Zhao
Qinwan Huang
Hongyun Zhou
Shifang Wan
Zhiwei Guan
Shanshan Li
Zhonghe Zhao
Source :
Journal of Microbiology and Biotechnology. 31:1295-06037
Publication Year :
2021
Publisher :
Journal of Microbiology and Biotechnology, 2021.

Abstract

Modified Renshen Wumei decoction (MRWD), a famous traditional Chinese medicine, is widely used for treating persistent diarrhoea. However, the mechanism by which MRWD regulates diarrhoea remains unknown. Therefore, we examined the protective effects of MRWD on intestinal barrier integrity in a diarrhoea model. In total, 48 male rats were randomly distributed to four treatment groups: the blank group (CK group), model group (MC group), Medilac-Vita group (MV group) and Chinese herb group (MRWD group). After a 10-day experiment, serum and colon samples were assessed. The diarrhoea index, pathological examination findings and change in D-lactate and diamine oxidase (DAO) contents illustrated that the induction of diarrhoea caused intestinal injury, which was ameliorated by MV and MRWD infusion. Metabolomics analysis identified several metabolites in the serum. Some critical metabolites, such as phosphoric acid, taurine, cortisone, leukotriene B4 and calcitriol, were found to be significantly elevated by MRWD infusion. Importantly, these differences correlated with mineral absorption and metabolism and peroxisome proliferator activated-receptors (PPARs) pathways. Moreover, it significantly increased the expression levels of TLR4, MyD88 and p-NF-κB p65 proteins and the contents of IL-1 and TNF-α, while the expression levels of occludin, claudin-1 and ZO-1 proteins decreased. These deleterious effects were significantly alleviated by MV and MRWD infusion. Our findings indicate that MRWD infusion helps alleviate diarrhoea, possibly by maintaining electrolyte homeostasis, improving the intestinal barrier integrity and inhibiting the TLR4/NF-κB axis.

Details

ISSN :
17388872 and 10177825
Volume :
31
Database :
OpenAIRE
Journal :
Journal of Microbiology and Biotechnology
Accession number :
edsair.doi.dedup.....6f0e39781926d2225c8cf3961474082d