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Up-regulation of an N-terminal truncated 3-hydroxy-3-methylglutaryl CoA reductase enhances production of essential oils and sterols in transgenic Lavandula latifolia
- Source :
- Plant Biotechnology Journal. 5:746-758
- Publication Year :
- 2007
- Publisher :
- Wiley, 2007.
-
Abstract
- Spike lavender (Lavandula latifolia) essential oil is widely used in the perfume, cosmetic, flavouring and pharmaceutical industries. Thus, modifications of yield and composition of this essential oil by genetic engineering should have important scientific and commercial applications. We generated transgenic spike lavender plants expressing the Arabidopsis thaliana HMG1 cDNA, encoding the catalytic domain of 3-hydroxy-3-methylglutaryl CoA reductase (HMGR1S), a key enzyme of the mevalonic acid (MVA) pathway. Transgenic T0 plants accumulated significantly more essential oil constituents as compared to controls (up to 2.1- and 1.8-fold in leaves and flowers, respectively). Enhanced expression of HMGR1S also increased the amount of the end-product sterols, beta-sitosterol and stigmasterol (average differences of 1.8- and 1.9-fold, respectively), but did not affect the accumulation of carotenoids or chlorophylls. We also analysed T1 plants derived from self-pollinated seeds of T0 lines that flowered after growing for 2 years in the greenhouse. The increased levels of essential oil and sterols observed in the transgenic T0 plants were maintained in the progeny that inherited the HMG1 transgene. Our results demonstrate that genetic manipulation of the MVA pathway increases essential oil yield in spike lavender, suggesting a contribution for this cytosolic pathway to monoterpene and sesquiterpene biosynthesis in leaves and flowers of the species.
- Subjects :
- Chlorophyll
Transgene
Arabidopsis
Stigmasterol
Lavandula latifolia
Mevalonic Acid
Plant Science
Mevalonic acid
Reductase
law.invention
chemistry.chemical_compound
law
Botany
Oils, Volatile
Arabidopsis thaliana
Carotenoid
Essential oil
chemistry.chemical_classification
biology
Phytosterols
food and beverages
Pigments, Biological
Plants, Genetically Modified
biology.organism_classification
Carotenoids
Sitosterols
Up-Regulation
Plant Leaves
Lavandula
chemistry
Biochemistry
Monoterpenes
Hydroxymethylglutaryl CoA Reductases
Sesquiterpenes
Agronomy and Crop Science
Biotechnology
Subjects
Details
- ISSN :
- 14677652 and 14677644
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Plant Biotechnology Journal
- Accession number :
- edsair.doi.dedup.....6f01f78f250fd2a1c45c10be2e9b5883