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Cellular targets and receptors for interleukin-6 I. In vivo and in vitro uptake of IL-6 in liver and hepatocytes
- Source :
- European Journal of Clinical Investigation. 20:366-370
- Publication Year :
- 2008
- Publisher :
- Wiley, 2008.
-
Abstract
- Interleukin-6 (IL-6) is a potent stimulator of the hepatic synthesis of acute-phase proteins. 125I-labelled IL-6 disappeared from the blood of rats with an overall half-time of about 1.5 min; 41% of the injected tracer dose was recovered in the liver by 15 min. The clearance was biphasic. The simultaneous injection of tracer and an excess of unlabelled IL-6 eliminated the initial rapid phase, and reduced the hepatic uptake to 14%. Light microscopic autoradiography showed 5% of the grains over non-hepatocytes, and 80% over hepatocytes, accumulating in areas around the bile canaliculi. Thereafter, degradation products accumulated in the bile. At 4 degrees C, isolated rat hepatocytes bound IL-6 with an apparent Kd of 39 pmol l-1 to a uniform class of 4500 receptors per cell with an apparent molar mass of 115-120 kg mol-1. The HepG2 human hepatocellular cell line bound IL-6 with an apparent Kd of 21 pmol l-1 to a uniform class of 1200 receptors per cell with an apparent molar mass of 155-160 kg mol-1. At 37 degrees C, both cell types endocytosed the bound ligand slowly, and degradation products appeared in the medium after a relatively long lag period (40 min in hepatocytes and 1 h in HepG2 cells).
- Subjects :
- Male
Cell type
Metabolic Clearance Rate
Clinical Biochemistry
Cell
In Vitro Techniques
Biology
Bone canaliculus
Biochemistry
Iodine Radioisotopes
In vivo
medicine
Animals
Humans
Receptors, Immunologic
Receptor
Interleukin-6
Rats, Inbred Strains
General Medicine
Ligand (biochemistry)
Receptors, Interleukin-6
Molecular biology
In vitro
Rats
Perfusion
Kinetics
medicine.anatomical_structure
Liver
Organ Specificity
Cell culture
Protein Binding
Subjects
Details
- ISSN :
- 13652362 and 00142972
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- European Journal of Clinical Investigation
- Accession number :
- edsair.doi.dedup.....6ef8e32d696fb7049b9ebd7cf46f19b2