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Loss of Vascular CD34 Results in Increased Sensitivity to Lung Injury
- Source :
- American journal of respiratory cell and molecular biology. 57(6)
- Publication Year :
- 2017
-
Abstract
- Survival during lung injury requires a coordinated program of damage limitation and rapid repair. CD34 is a cell surface sialomucin expressed by epithelial, vascular, and stromal cells that promotes cell adhesion, coordinates inflammatory cell recruitment, and drives angiogenesis. To test whether CD34 also orchestrates pulmonary damage and repair, we induced acute lung injury in wild-type (WT) and Cd34-/- mice by bleomycin administration. We found that Cd34-/- mice displayed severe weight loss and early mortality compared with WT controls. Despite equivalent early airway inflammation to WT mice, CD34-deficient animals developed interstitial edema and endothelial delamination, suggesting impaired endothelial function. Chimeric Cd34-/- mice reconstituted with WT hematopoietic cells exhibited early mortality compared with WT mice reconstituted with Cd34-/- cells, supporting an endothelial defect. CD34-deficient mice were also more sensitive to lung damage caused by influenza infection, showing greater weight loss and more extensive pulmonary remodeling. Together, our data suggest that CD34 plays an essential role in maintaining vascular integrity in the lung in response to chemical- and infection-induced tissue damage.
- Subjects :
- 0301 basic medicine
Pulmonary and Respiratory Medicine
Pathology
medicine.medical_specialty
Stromal cell
Angiogenesis
Clinical Biochemistry
Cell
CD34
Antigens, CD34
Pulmonary Edema
Biology
Lung injury
Bleomycin
03 medical and health sciences
chemistry.chemical_compound
Mice
medicine
Animals
Cell adhesion
Molecular Biology
Mice, Knockout
Lung
Cell Biology
Lung Injury
030104 developmental biology
medicine.anatomical_structure
chemistry
Airway Remodeling
Endothelium, Vascular
Subjects
Details
- ISSN :
- 15354989
- Volume :
- 57
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- American journal of respiratory cell and molecular biology
- Accession number :
- edsair.doi.dedup.....6ef6847187415479449fc8814027a264