Back to Search
Start Over
A sensitised RNAi screen reveals a ch-TOG genetic interaction network required for spindle assembly
- Source :
- Scientific Reports
- Publication Year :
- 2014
-
Abstract
- How multiple spindle assembly pathways are integrated to drive bipolar spindle assembly is poorly understood. We performed an image-based double RNAi screen to identify genes encoding Microtubule-Associated Proteins (MAPs) that interact with the highly conserved ch-TOG gene to regulate bipolar spindle assembly in human cells. We identified a ch-TOG centred network of genetic interactions which promotes centrosome-mediated microtubule polymerisation, leading to the incorporation of microtubules polymerised by all pathways into a bipolar structure. Our genetic screen also reveals that ch-TOG maintains a dynamic microtubule population, in part, through modulating HSET activity. ch-TOG ensures that spindle assembly is robust to perturbation but sufficiently dynamic such that spindles can explore a diverse shape space in search of structures that can align chromosomes.
- Subjects :
- CHROMOSOME CONGRESSION
Population
CELL-DIVISION
Kinesins
Mitosis
PLUS-END
Spindle Apparatus
Biology
Microtubules
Article
Microtubule
Tubulin
SISTER-CHROMATID COHESION
Cluster Analysis
Humans
Gene Regulatory Networks
RNA, Small Interfering
education
XENOPUS EGG EXTRACTS
INTERACTION MAP
Gene
education.field_of_study
Science & Technology
Multidisciplinary
Genetic interaction
MICROTUBULE-ASSOCIATED PROTEIN
Gene Expression Profiling
Dyneins
Epistasis, Genetic
Kinesin
Corrigenda
Chromatin
Cell biology
Multidisciplinary Sciences
Rnai screen
Shape space
MITOTIC-SPINDLE
Science & Technology - Other Topics
RNA Interference
SIGNALING NETWORKS
Microtubule-Associated Proteins
CROSS-LINKS MICROTUBULES
Genetic screen
Protein Binding
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Scientific reports
- Accession number :
- edsair.doi.dedup.....6ef4ae27c15ab490ba5f7d095017dd21