Supplemental Figure 1 from The Prolyl Isomerase Pin1 Is a Novel Target of 6,7,4′-Trihydroxyisoflavone for Suppressing Esophageal Cancer Growth

6,7,4'-THIF does not bind to FKBP or cyclophilin A. The interaction between 6,7,4'-THIF and FKBP or cyclophilin A was confirmed using 6,7,4'-THIF-Sepharose 4B beads. 6,7,4'-THIF-Sepharose 4B beads were incubated with each cell lysate overnight. Total experimental procedures are described in Materials and Methods.