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Physicochemical Characterization of Hizikia fusiforme Polysaccharide and Its Hypoglycemic Activity via Mediating InsulināStimulated Blood Glucose Utilization of Skeletal Muscle in Type 2 Diabetic Rats
- Source :
- Chemistry & Biodiversity. 17
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- In the current study, a functional polysaccharide fraction (HFP) was obtained from Hizikia fusiforme by ultrasound-assisted enzymatic extraction, and its structural characterization and hypoglycemic activity and potential molecular mechanism were investigated. The results indicated that HFP with high uronic acid was a heterogeneous polysaccharide composed of six monosaccharides. Congo red test explained that HFP had no triple helix conformation. AFM analysis revealed that HFP was spherical particle with flame-like aggregates and multiple strands closely arranged. Rheological analysis showed that HFP exhibited shear-thinning flow behavior. HFP significantly ameliorated diabetes-related symptoms and serum profiles and increased muscle glycogen storage in rats. HFP administration at 400 mg/kg body weight/day displayed greater advantages than metformin in controlling the levels of fasting blood glucose, triglyceride (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bile acid (TBA) of diabetic rats. Intervention of HFP up-regulated markedly the expression of AMPK-Ī±, GLUT4, PI3K and Akt in skeletal muscle of diabetic rats at the mRNA and protein levels, revealing hypoglycemic effects of HFP may be related closely to improving insulin resistance and mitochondrial function of skeletal muscle.
- Subjects :
- Blood Glucose
Male
medicine.medical_specialty
medicine.drug_class
medicine.medical_treatment
Bioengineering
Polysaccharide
01 natural sciences
Biochemistry
Diabetes Mellitus, Experimental
Rats, Sprague-Dawley
chemistry.chemical_compound
Insulin resistance
Polysaccharides
Internal medicine
medicine
Animals
Hypoglycemic Agents
Muscle, Skeletal
Molecular Biology
chemistry.chemical_classification
Glycogen
Triglyceride
biology
Bile acid
Chemistry, Physical
Plant Extracts
010405 organic chemistry
Insulin
Sargassum
Skeletal muscle
General Chemistry
General Medicine
medicine.disease
Mitochondria
Rats
0104 chemical sciences
010404 medicinal & biomolecular chemistry
Endocrinology
medicine.anatomical_structure
Diabetes Mellitus, Type 2
chemistry
biology.protein
Molecular Medicine
Insulin Resistance
GLUT4
Subjects
Details
- ISSN :
- 16121880 and 16121872
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- Chemistry & Biodiversity
- Accession number :
- edsair.doi.dedup.....6ed583fc6760f77fabb1e4c65965a5f3