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Role of Nicotinic Acetylcholine Receptor α3 and α7 Subunits in Detrusor Overactivity Induced by Partial Bladder Outlet Obstruction in Rats
- Source :
- International Neurourology Journal, Vol 19, Iss 1, Pp 12-18 (2015), International Neurourology Journal
- Publication Year :
- 2015
- Publisher :
- Korean Continence Society, 2015.
-
Abstract
- Purpose: To investigate the role of α3 and α7 nicotinic acetylcholine receptor subunits (nAChRs) in the bladder, using a rat model with detrusor overactivity induced by partial bladder outlet obstruction (BOO). Methods: Forty Sprague-Dawley rats were used: 10 were sham-operated (control group) and 30 were observed for 3 weeks after partial BOO. BOO-induced rats were further divided into 3 groups: Two groups of 10 rats each received intravesicular infusions with hexamethonium (HM group; n=10) or methyllycaconitine (MLC group; n=10), which are antagonists for α3 and α7 nAChRs, respectively. The remaining BOO-induced rats received only saline infusion (BOO group; n=10). Based on the contraction interval measurements using cystometrogram, the contraction pressure and nonvoiding bladder contractions were compared between the control and the three BOO-induced groups. Immunofluorescent staining and Western blotting were used to analyze α3 and α7 nAChRs levels. Results: The contraction interval of the MLC group was higher than that of the BOO group (P
- Subjects :
- Detrusor muscle
medicine.medical_specialty
Contraction (grammar)
Urology
Alpha7 Nicotinic Acetylcholine Receptor
lcsh:RC870-923
urologic and male genital diseases
Bladder outlet obstruction
chemistry.chemical_compound
medicine
Urothelium
Methyllycaconitine
Urinary Bladder, Overactive
business.industry
Urinary bladder neck obstruction
lcsh:Diseases of the genitourinary system. Urology
medicine.disease
Urinary Bladder Neck Obstruction
Nicotinic acetylcholine receptor
medicine.anatomical_structure
Neurology
chemistry
Original Article
Hexamethonium
Neurology (clinical)
business
Subjects
Details
- ISSN :
- 20936931
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- International Neurourology Journal
- Accession number :
- edsair.doi.dedup.....6ecb93bffc26d85c637a49ccad48db1c