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Insulin-like peptides in Spodoptera littoralis (Lepidoptera): Detection, localization and identification
- Source :
- General and Comparative Endocrinology. 153:72-79
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- Insulin is an extensively studied peptide hormone in mammals. However, insulin is not restricted to vertebrates, but has also been identified in invertebrates, among whom several insect species. These insulin-like peptides (ILPs) show structural and-at least some-functional homology with mammalian insulin and act through a conserved pathway. Yet many aspects of insulin function in insects remain to be unveiled. We analyzed the presence of ILPs in the cotton leafworm, Spodoptera littoralis, at two levels: (1) cellular localization of ILPs in whole tissues of the central nervous system from S. littoralis, and (2) detection and identification of ILPs at nucleotide level. To our knowledge, nothing about the presence of ILPs in S. littoralis has been described so far. By whole mount in situ immunolocalization, we localized bombyxin-like material in S. littoralis in four pairs of pars intercerebralis cells and in the corpus cardiacum-corpus allatum complexes. In addition, we have cloned two different S. littoralis ILP precursor cDNAs by a combination of PCR and RAcE. The corresponding precursor polypeptides ('Sl-ILPP1' and 'Sl-ILPP2') show significant sequence homology with precursors for bombyxin and other bombyxin-related peptides. Our results strongly suggest that the S. littoralis ILPs belong to the category of bombyxin-analogs. ispartof: General and Comparative Endocrinology vol:153 issue:1 pages:72-9 ispartof: location:ENGLAND, Univ Sheffield, Sheffield status: published
- Subjects :
- PROTHORACICOTROPIC HORMONE
medicine.medical_treatment
insulin-related peptides
RECEPTOR HOMOLOG
Insect
LOCUSTA-MIGRATORIA
Homology (biology)
Endocrinology
Insulin
Tissue Distribution
Cloning, Molecular
Cellular localization
LIFE-SPAN
media_common
Whole mount
biology
Brain
Spodoptera littoralis
insulin-like peptides
BOMBYX-MORI
CLEAVAGE SITES
Biochemistry
cotton leafworm
Insect Hormones
Life Sciences & Biomedicine
medicine.medical_specialty
media_common.quotation_subject
Molecular Sequence Data
SAMIA-CYNTHIA-RICINI
NEUROSECRETORY-CELLS
Spodoptera
Peptide hormone
Lepidoptera genitalia
Endocrinology & Metabolism
Internal medicine
medicine
Animals
Amino Acid Sequence
Science & Technology
Base Sequence
Sequence Homology, Amino Acid
Neuropeptides
biology.organism_classification
DROSOPHILA-MELANOGASTER
SIGNAL PEPTIDES
bombyxin
Animal Science and Zoology
Subjects
Details
- ISSN :
- 00166480
- Volume :
- 153
- Database :
- OpenAIRE
- Journal :
- General and Comparative Endocrinology
- Accession number :
- edsair.doi.dedup.....6ec7e66ad4251ba5d73bd4264815555c