Back to Search Start Over

Protein-induced metamorphosis of unilamellar lipid vesicles to multilamellar hybrid vesicles

Authors :
Inhye Kim
Jong Min Yuk
Seo Yeon Shin
Kunmo Koo
Eunji Lee
Moon Young Yang
Sung Duk Jo
Kyung Mok Park
Yoon Sung Nam
Bon Il Koo
Source :
Journal of Controlled Release. 331:187-197
Publication Year :
2021
Publisher :
Elsevier BV, 2021.

Abstract

Protein encapsulation into nanocarriers has been extensively studied to improve the efficacy and stability of therapeutic proteins. However, the chemical modification of proteins or new synthetic carrier materials are essential to achieve a high encapsulation efficiency and structural stability of proteins, which hinders their clinical applications. New strategies to physically incorporate proteins into nanocarriers feasible for clinical uses are required to overcome the current limitation. Here we report the spontaneous protein-induced reorganization of 'pre-formed' unilamellar lipid vesicles to efficiently incorporate proteins within multilamellar protein-lipid hybrid vesicles without chemical modification. Epidermal growth factor (EGF) binds to the surface of cationic unilamellar lipid vesicles and induces layer-by-layer self-assembly of the vesicles. The protein is spontaneously entrapped in the interstitial layers of a multilamellar structure with extremely high loading efficiency, ~99%, through polyionic interactions as predicted by molecular dynamics simulation. The loaded protein exhibits much higher structural, chemical, and biological stability compared to free protein. The method is also successfully applied to several other proteins. This work provides a promising method for the highly efficient encapsulation of therapeutic proteins into multilamellar lipid vesicles without the use of specialized instruments, high energy, coupling agents, or organic solvents.

Details

ISSN :
01683659
Volume :
331
Database :
OpenAIRE
Journal :
Journal of Controlled Release
Accession number :
edsair.doi.dedup.....6ec126cabe656ed760e2032a31ed1ea0