Blastic Transformation Induced on the Lymphocytes «in vitro» by an Exudate of Reticulum Cell Sarcoma

The blood lymphocyte culture «in vitro» has been employed in order to investigate (a) the presence of neoplastic antigens in reticulum cell sarcoma exudates, and (b) the ability of patients with reticulum cell proliferative diseases (reticulum cell sarcoma, reticulum cell reticulosis) to give immune reactions to such antigens. Lymphocytes from a patient with reticulum cell reticulosis showed a 7.3% transformation to blast cells in the presence of the pleural exudate from a reticulum cell sarcoma patient. However, this same exudate did not induce transformation of lymphocytes from two other patients with reticulum cell sarcoma nor of normal lymphocytes. Lymphocytes from the exudate donor patient did show a small percentage transformation in the presence as well as in the absence of the exudate (2.3, and 2.1%, respectively). With the purpose of identification of the transformating factor, fractionation studies were performed yielding a carbohydrate-rich, partially dialyzable mucoprotein. The conclusion is drawn that lymphocytes from the patient with reticulum cell reticulosis were immunologically reactive to a neoplastic antigen contained in the reticulum cell sarcoma exudate, and possibly produced by the proliferating reticulum cells.