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Chlamydia trachomatis Intercepts Golgi-Derived Sphingolipids through a Rab14-Mediated Transport Required for Bacterial Development and Replication
- Source :
- PLoS ONE, Vol 5, Iss 11, p e14084 (2010), PLoS ONE
- Publication Year :
- 2010
- Publisher :
- Public Library of Science (PLoS), 2010.
-
Abstract
- Chlamydia trachomatis are obligate intracellular bacteria that survive and replicate in a bacterial-modified phagosome called inclusion. As other intracellular parasites, these bacteria subvert the phagocytic pathway to avoid degradation in phagolysosomes and exploit trafficking pathways to acquire both energy and nutrients essential for their survival. Rabs are host proteins that control intracellular vesicular trafficking. Rab14, a Golgi-related Rab, controls Golgi to endosomes transport. Since Chlamydia establish a close relationship with the Golgi apparatus, the recruitment and participation of Rab14 on inclusion development and bacteria growth were analyzed. Time course analysis revealed that Rab14 associated with inclusions by 10 h post infection and was maintained throughout the entire developmental cycle. The recruitment was bacterial protein synthesis-dependent but independent of microtubules and Golgi integrity. Overexpression of Rab14 dominant negative mutants delayed inclusion enlargement, and impaired bacteria replication as determined by IFU. Silencing of Rab14 by siRNA also decreased bacteria multiplication and infectivity. By electron microscopy, aberrant bacteria were observed in cells overexpressing the cytosolic negative Rab14 mutant. Our results showed that Rab14 facilitates the delivery of sphingolipids required for bacterial development and replication from the Golgi to chlamydial inclusions. Novel anti-chlamydial therapies could be developed based on the knowledge of how bacteria subvert host vesicular transport events through Rabs manipulation.
- Subjects :
- Endosome
Green Fluorescent Proteins
Immunology/Innate Immunity
Golgi Apparatus
lcsh:Medicine
Chlamydia trachomatis
Biology
medicine.disease_cause
Microbiology
Infectious Diseases/Bacterial Infections
Cell Biology/Microbial Growth and Development
symbols.namesake
Bacterial Proteins
Microscopy, Electron, Transmission
Cell Biology/Membranes and Sorting
Microtubule
Biochemistry/Cell Signaling and Trafficking Structures
Infectious Diseases/Sexually Transmitted Diseases
medicine
Humans
Immunology/Cellular Microbiology and Pathogenesis
lcsh:Science
Phagosome
Inclusion Bodies
Sphingolipids
Microscopy, Confocal
Microbiology/Microbial Growth and Development
Multidisciplinary
Intracellular parasite
lcsh:R
Biological Transport
Golgi apparatus
Microbiology/Immunity to Infections
Cell biology
Vesicular transport protein
rab GTP-Binding Proteins
Host-Pathogen Interactions
symbols
RNA Interference
lcsh:Q
Rab
Microbiology/Cellular Microbiology and Pathogenesis
HeLa Cells
Research Article
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....6e977aa3157747e3c5a6f7bb4ba2244a