Additional file 5: of Albugo-imposed changes to tryptophan-derived antimicrobial metabolite biosynthesis may contribute to suppression of non-host resistance to Phytophthora infestans in Arabidopsis thaliana

AlNc14 suppresses benzo-(1,2,3)-thiadiazole-7-carbothioic acid (BTH) and salicylic acid (SA) induction of PR1. To visualize reporter gene induction and pathogen growth in the same leaf, leaves were collected and stained with magenta-GUS to reveal GUS activity, followed by trypan blue to reveal pathogen growth. Leaves of Col-0 pro(PR1)::GUS were previously inoculated with water or AlNc14 (13 dpi) and infiltrated with DMSO (mock), BTH (200 μM) or SA (200 μM) for 8 hours, then stained and examined with a microscope. The percentage of each leaf stained with GUS was determined using ImageJ. (A) Open circles represent mean ± SE of the raw data (percentage of leaf stained) of three independent biological replicates with between two and seven technical replicates per biological replicate (bars left to right n = 10, 12, 13, 15, 14 and 16). (B) Open circles represent mean ± SE of the transformed data (arcsine square root transformation followed by log10 transformation) of three independent biological replicates with between two and seven technical replicates per biological replicate (bars left to right n = 10, 12, 13, 15, 14 and 16). Different letters indicate significant differences P