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Reversed T Cell Receptor Docking on a Major Histocompatibility Class I Complex Limits Involvement in the Immune Response
- Source :
- Immunity. 45:749-760
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- The anti-viral T cell response is drawn from the naive T cell repertoire. During influenza infection, the CD8+ T cell response to an H-2Db-restricted nucleoprotein epitope (NP366) is characterized by preferential expansion of T cells bearing TRBV13+ T cell receptors (TCRs) and avoidance of TRBV17+ T cells, despite the latter dominating the naive precursor repertoire. We found two TRBV17+ TCRs that bound H-2Db-NP366 with a 180° reversed polarity compared to the canonical TCR-pMHC-I docking. The TRBV17 β-chain dominated the interaction and, whereas the complementarity determining region-3 (CDR3) loops exclusively mediated contacts with the MHC-I, peptide specificity was attributable to germline-encoded recognition. Nevertheless, the TRBV17+ TCR exhibited moderate affinity toward H-2Db-NP366 and was capable of signal transduction. Thus, the naive CD8+ T cell pool can comprise TCRs adopting reversed pMHC-I docking modes that limit their involvement in the immune response.
- Subjects :
- Models, Molecular
0301 basic medicine
T cell
Immunology
Receptors, Antigen, T-Cell
chemical and pharmacologic phenomena
CD8-Positive T-Lymphocytes
Biology
Crystallography, X-Ray
Major histocompatibility complex
Jurkat cells
Epitope
Cell Line
Epitopes
Jurkat Cells
Mice
03 medical and health sciences
0302 clinical medicine
Antigen
Cell Line, Tumor
medicine
Animals
Humans
Immunology and Allergy
Histocompatibility Antigens Class I
T-cell receptor
R1
Cell biology
Mice, Inbred C57BL
HEK293 Cells
030104 developmental biology
Infectious Diseases
medicine.anatomical_structure
biology.protein
Female
Signal transduction
CD8
030215 immunology
Subjects
Details
- ISSN :
- 10747613
- Volume :
- 45
- Database :
- OpenAIRE
- Journal :
- Immunity
- Accession number :
- edsair.doi.dedup.....6e8cd559452534fb0ce2573703c35b7a