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Cell-cycle regulation of cohesin stability along fission yeast chromosomes
- Source :
- EMBO Journal, EMBO Journal, EMBO Press, 2007, 27 (1), pp.111-121. ⟨10.1038/sj.emboj.7601955⟩
- Publication Year :
- 2007
- Publisher :
- Nature Publishing Group, 2007.
-
Abstract
- International audience; Sister chromatid cohesion is mediated by cohesin, but the process of cohesion establishment during S-phase is still enigmatic. In mammalian cells, cohesin binding to chromatin is dynamic in G1, but becomes stabilized during S-phase. Whether the regulation of cohesin stability is integral to the process of cohesion establishment is unknown. Here, we provide evidence that fission yeast cohesin also displays dynamic behavior. Cohesin association with G1 chromosomes requires continued activity of the cohesin loader Mis4/Ssl3, suggesting that repeated loading cycles maintain cohesin binding. Cohesin instability in G1 depends on wpl1, the fission yeast ortholog of mammalian Wapl, suggestive of a conserved mechanism that controls cohesin stability on chromosomes. wpl1 is nonessential, indicating that a change in wpl1-dependent cohesin dynamics is dispensable for cohesion establishment. Instead, we find that cohesin stability increases at the time of S-phase in a reaction that can be uncoupled from DNA replication. Hence, cohesin stabilization might be a pre-requisite for cohesion establishment rather than its consequence.
- Subjects :
- G2 Phase
Chromosomal Proteins, Non-Histone
[SDV]Life Sciences [q-bio]
MESH: Cell Cycle
Cell Cycle Proteins
MESH: G1 Phase
General Biochemistry, Genetics and Molecular Biology
Article
MESH: Chromatin
S Phase
Chromosome segregation
03 medical and health sciences
0302 clinical medicine
MESH: Cell Cycle Proteins
MESH: Chromosomal Proteins, Non-Histone
Schizosaccharomyces
MESH: Protein Binding
Molecular Biology
[SDV.BC] Life Sciences [q-bio]/Cellular Biology
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
0303 health sciences
General Immunology and Microbiology
biology
Cohesin
General Neuroscience
Cell Cycle
DNA replication
MESH: S Phase
G1 Phase
Nuclear Proteins
Cell cycle
biology.organism_classification
MESH: Chromosomes, Fungal
Chromatin
Cell biology
Establishment of sister chromatid cohesion
MESH: G2 Phase
MESH: Schizosaccharomyces
Schizosaccharomyces pombe
Separase
biological phenomena, cell phenomena, and immunity
Chromosomes, Fungal
MESH: Sister Chromatid Exchange
MESH: Nuclear Proteins
Sister Chromatid Exchange
030217 neurology & neurosurgery
Protein Binding
Subjects
Details
- Language :
- English
- ISSN :
- 02614189 and 14602075
- Database :
- OpenAIRE
- Journal :
- EMBO Journal, EMBO Journal, EMBO Press, 2007, 27 (1), pp.111-121. ⟨10.1038/sj.emboj.7601955⟩
- Accession number :
- edsair.doi.dedup.....6e89e2fa22285349d3552caf15a27aed