Back to Search
Start Over
Expression of bcl-2 protein and APO-1 (Fas antigen) in the lung tissue from patients with idiopathic pulmonary fibrosis
- Source :
- Microscopy research and technique. 38(5)
- Publication Year :
- 1997
-
Abstract
- The fibrotic process of idiopathic pulmonary fibrosis (IPF) is considered to be the consequence of an exaggerated response to an inflammatory lung injury. In a previous report, except for the response to PG-E2, we found no difference in the proliferative profiles of lung fibroblasts between IPF patients and healthy subjects [Mio et al. (1992) Chest, 102:832-837]. In the present study, we hypothesized that lung fibroblasts from IPF patients would not undergo apoptosis as observed in the normal repair process. Additionally, we focused on the protooncogene bcl-2 which prevents apoptosis and the APO-1 (Fas antigen) which induces apoptosis. In order to explore this question, we used immunohistochemical staining to investigate whether apoptotic markers are expressed on lung parenchymal cells of IPF patients obtained by open lung biopsy. Bcl-2 protein was expressed on mononuclear cells in the mantle zone of lymphoid follicules and smooth muscle cells, but it was not expressed on other parenchymal cells. Apo-1 was expressed on epithelial cells, some germinal center cells, and many parenchymal cells including smooth muscle cells, fibrocytes, and myofibroblasts in patients with IPF, findings of which are fundamentally the same as those in normal subjects. Although we could not find any abnormality of lung fibroblasts in IPF patients, the positive staining with anti-Bcl-2 monoclonal antibody and anti-Fas (anti-APO-1) monoclonal antibody in lung lymphoid follicules suggests the continuous activation of B lymphocytes localized in the lung parenchyma in patients with IPF. The role of apoptosis in fibrosis should be further examined.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Histology
Pulmonary Fibrosis
Apoptosis
Biology
Lung injury
Idiopathic pulmonary fibrosis
Antigen
Fibrosis
Parenchyma
medicine
Humans
fas Receptor
Instrumentation
Lung
Aged
Germinal center
respiratory system
Middle Aged
medicine.disease
Immunohistochemistry
respiratory tract diseases
Medical Laboratory Technology
medicine.anatomical_structure
Proto-Oncogene Proteins c-bcl-2
Female
Anatomy
Subjects
Details
- ISSN :
- 1059910X
- Volume :
- 38
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Microscopy research and technique
- Accession number :
- edsair.doi.dedup.....6e850cb233279de297b7fe1e8e30f60a