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Prognostic Value of Intratumoral Interferon Gamma Messenger RNA Expression in Invasive Cervical Carcinomas
- Source :
- JNCI: Journal of the National Cancer Institute. 90:287-294
- Publication Year :
- 1998
- Publisher :
- Oxford University Press (OUP), 1998.
-
Abstract
- Background The production of the cytokine interferon gamma (IFN gamma) by activated peripheral blood mononuclear cells may be reduced in patients with invasive cervical carcinoma. This study was designed to assess the prognostic value of intratumoral IFN gamma messenger RNA (mRNA) levels in such patients. Methods Biopsy specimens of primary cervical lesions were obtained from 27 patients with invasive squamous cell carcinoma before they received any therapy. Two prognostic groups were considered: 1) a group of 14 patients who had no apparent disease recurrence and who were alive 2 years after diagnosis (good-prognosis group) and 2) a group of 13 patients who had disease recurrence or died during the 2-year follow-up (poor-prognosis group). A competitive reverse transcription-polymerase chain reaction assay was used to measure levels of IFN gamma and beta actin mRNA. The expression of human leukocyte antigen (HLA) class II proteins (which is stimulated by IFN gamma) in tumor cells was studied by immunostaining. Results Tumor specimens from all 14 patients in the good-prognosis group contained more than 10(3) IFN gamma mRNA copies per 5 x 10(5) beta actin mRNA copies, whereas tumor specimens from only six of the 13 patients in the poor-prognosis group contained this level of IFN gamma mRNA (two-sided P = .006). No clear relationship was observed between levels of IFN gamma mRNA and T-cell or natural killer cell infiltration in tumors; however, a statistically significant association was observed between HLA class II expression on tumor cells and IFN gamma mRNA levels (two-sided P = .01). Conclusions A subgroup of poor-prognosis cervical carcinoma patients who have low levels of intratumoral IFN gamma mRNA was identified.
- Subjects :
- Cancer Research
Pathology
medicine.medical_specialty
Interferon type II
medicine.medical_treatment
Uterine Cervical Neoplasms
Biology
Polymerase Chain Reaction
Peripheral blood mononuclear cell
Natural killer cell
Interferon-gamma
Predictive Value of Tests
Biopsy
medicine
Carcinoma
Humans
Neoplasm Invasiveness
Interferon gamma
RNA, Messenger
RNA, Neoplasm
medicine.diagnostic_test
Recombinant Interferon Gamma
Prognosis
medicine.disease
Gene Expression Regulation, Neoplastic
Cytokine
medicine.anatomical_structure
Oncology
Carcinoma, Squamous Cell
Female
DNA Probes
medicine.drug
Subjects
Details
- ISSN :
- 14602105 and 00278874
- Volume :
- 90
- Database :
- OpenAIRE
- Journal :
- JNCI: Journal of the National Cancer Institute
- Accession number :
- edsair.doi.dedup.....6e832557abe98632f042316616a0566e