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The Effect of Shiga Toxin on Weibel-Palade Bodies in Primary Human Endothelial Cells
- Source :
- Nephron Extra, Vol 4, Iss 2, Pp 101-107 (2014), Nephron Extra, 4(2), 101. S. Karger AG, Nephron Extra, Nephron Extra, 4, 101-7, Nephron Extra, 4, 2, pp. 101-7
- Publication Year :
- 2014
- Publisher :
- S. Karger AG, 2014.
-
Abstract
- Background/Aims: Diarrhea-associated hemolytic uremic syndrome is associated with the presence of Shiga toxin (Stx1, Stx2 and several variants) in the circulation. The aim of this study is to examine the possible triggering effect of Stx1 on the exocytosis of Weibel-Palade bodies (WPbs). Methods: Cultured human umbilical venous endothelial cells (HUVECs) and glomerular microvascular endothelial cells (GMVECs) were stimulated by thrombin and Stx1 in both static and flowing conditions. The amount of secreted von Willebrand factor (VWF) in the supernatant as well as the remaining intracellular fraction was determined. Results: In HUVECs and in 2 out of 4 GMVECs, the stimulation of Stx1 in flow at 1 dyne/cm2 resulted in a decrease of intracellular VWF. This is contrary to the results of Stx1 applied in static conditions. At a higher flow rate of 5 dyne/cm2, no effect in GMVECs was observed. Conclusion: Stx1 can contribute, via an effect on WPbs, to the exocytosis of WPbs in flow conditions in HUVECs and probably in GMVECs. This results in the release of VWF, suggesting an initiating role of the coagulation system in the pathogenesis.
- Subjects :
- Original Paper
biology
Endothelial cells
Other Research Radboud Institute for Molecular Life Sciences [Radboudumc 0]
Stimulation
Shiga toxin
von Willebrand factor
lcsh:Diseases of the genitourinary system. Urology
lcsh:RC870-923
Exocytosis
Microbiology
Cell biology
Pathogenesis
fluids and secretions
Thrombin
Von Willebrand factor
Nephrology
cardiovascular system
biology.protein
medicine
Weibel–Palade body
Weibel-Palade bodies
Intracellular
medicine.drug
Subjects
Details
- ISSN :
- 16645529
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- Nephron Extra
- Accession number :
- edsair.doi.dedup.....6e812d2a1f2055287eb78fa9ceab263b