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Quantitative Polymerase Chain Reaction Profiling of Immunomarkers in Rejecting Kidney Allografts for Predicting Response to Steroid Treatment
- Source :
- Transplantation, 94(6), 596-602
- Publication Year :
- 2012
-
Abstract
- BACKGROUND Steroid-resistant acute rejection is a risk factor for inferior renal allograft outcome. METHODS From 873 kidney transplant recipients (1995-2005), 108 patients with a first rejection episode were selected for study using strict inclusion criteria and clinical endpoint definition. We aimed to predict response to corticosteroid treatment using gene expression of 65 transcripts. These reflect cytokines, chemokines, and surface and activation markers of various cell types including T cells, macrophages, B cells, and granulocytes. Steroid resistance (40% of the patients) was defined as requirement for antithymocyte globulin treatment within 2 weeks after corticosteroid treatment. RESULTS None of the clinical and histomorphologic parameters showed a significant association with response to treatment. Univariate logistic regression analysis resulted in 11 messenger RNA markers, including T-cell-related transcripts CD25, lymphocyte activation gene-3, Granzyme B, and interleukin-10, and macrophage-specific transcripts mannose receptor and S100 calcium-binding protein A9, which significantly discriminated steroid resistant from steroid-responsive rejections (P
- Subjects :
- Genetic Markers
Graft Rejection
Male
medicine.medical_specialty
Time Factors
Biopsy
Drug Resistance
Kidney biopsy
Logistic regression
Real-Time Polymerase Chain Reaction
Gastroenterology
Risk Assessment
Kidney transplantation
Acute allograft rejection
Adrenal Cortex Hormones
Predictive Value of Tests
Risk Factors
Internal medicine
medicine
Clinical endpoint
Odds Ratio
Humans
IL-2 receptor
Netherlands
Retrospective Studies
Transplantation
business.industry
Graft Survival
Reproducibility of Results
Odds ratio
Middle Aged
Confidence interval
Granzyme B
Real-time polymerase chain reaction
Logistic Models
Treatment Outcome
Gene Expression Regulation
Predictive value of tests
Immunology
Acute Disease
Multivariate Analysis
Female
Gene expression
business
Steroid resistance
Immunosuppressive Agents
Immunosuppression
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Transplantation, 94(6), 596-602
- Accession number :
- edsair.doi.dedup.....6e7d3f60661046654beace6f123e6b86