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Identification of novel HLA-A11-restricted T-cell epitopes in the Ebola virus nucleoprotein
- Source :
- Microbes and Infection. 21:56-62
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- The Ebola virus (EBOV) is a very contagious virus that is highly fatal in humans and animals. The largest epidemic was in West Africa in 2014, in which over 11,000 people died. However, to date, there are no licensed vaccines against it. Studies show that CD4+ and CD8+ T-cell responses, especially cytotoxic T-lymphocyte (CTL) responses, play key roles in protecting individuals from EBOV infection. Since HLA-restricted epitope vaccines are likely to be effective and safe immunization strategies for infectious diseases, the present study screened for CTL epitopes in the EBOV-nucleoprotein that are restricted by HLA-A11 (a common allele in Chinese people). Predictive computer analysis of the amino-acid sequence of EBOV-nucleoprotein identified ten putative HLA-A11-restricted epitopes. ELISPOT assay of immunized HLA-A11/DR1 transgenic mice showed that five (GR-9, VR-9, EK-9, PK-9, and RK-9) induced effective CTL responses. Additional epitope analyses will aid the design of epitope vaccines against EBOV.
- Subjects :
- 0301 basic medicine
030106 microbiology
Immunology
Epitopes, T-Lymphocyte
Mice, Transgenic
Human leukocyte antigen
Biology
Antibodies, Viral
medicine.disease_cause
Microbiology
Virus
Epitope
Adenoviridae
HLA-A11 Antigen
Mice
03 medical and health sciences
medicine
Animals
Humans
Amino Acid Sequence
Vaccines, Synthetic
Ebola virus
ELISPOT
Viral Vaccines
Hemorrhagic Fever, Ebola
Nucleocapsid Proteins
Ebolavirus
Virology
Nucleoprotein
Mice, Inbred C57BL
CTL
030104 developmental biology
Infectious Diseases
Immunization
Female
T-Lymphocytes, Cytotoxic
Subjects
Details
- ISSN :
- 12864579
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Microbes and Infection
- Accession number :
- edsair.doi.dedup.....6e799ba1ea2cb4577b1ab795356c3910