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Pulmonary delivery of tobramycin-loaded nanostructured lipid carriers for Pseudomonas aeruginosa infections associated with cystic fibrosis
- Source :
- International Journal of Pharmaceutics. 498:263-273
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Among the pathogens that affect cystic fibrosis (CF) patients, Pseudomonas aeruginosa is the most prevalent. As a way to fight against this infection, nanotechnology has emerged over the last decades as a promising alternative to overcome resistance to antibiotics in infectious diseases. The goal of this work was to elaborate and characterize lipid nanoparticles for pulmonary delivery of tobramycin. Tobramycin-loaded nanostructured lipid carriers (Tb-NLCs) were prepared by hot melt homogenization technique. In addition, nanoparticles labeled with infrared dye (IR-NLCs) were used to investigate their in vivo performance after pulmonary administration. Tb-NLCs displayed a mean diameter size around 250 nm, high drug encapsulation (93%) and sustained release profile. Tb-NLCs showed to be active against clinically isolated P. aeruginosa. Moreover, Tb-NLCs did not decrease cell viability and were able to overcome an artificial mucus barrier in the presence of mucolytics agents. During the in vivo assay, IR-NLCs were administered to several mice by the intratracheal route using a Penn Century device. Next, the biodistribution of the nanoparticles was analyzed at different time points showing a wide nanosystem distribution in the lungs. Altogether, tobramycin-loaded NLCs seem to us an encouraging alternative to the currently available CF therapies.
- Subjects :
- Biodistribution
Cystic Fibrosis
Pharmaceutical Science
02 engineering and technology
medicine.disease_cause
030226 pharmacology & pharmacy
Cystic fibrosis
Microbiology
Mice
03 medical and health sciences
Drug Delivery Systems
0302 clinical medicine
In vivo
Administration, Inhalation
medicine
Tobramycin
Animals
Humans
Pseudomonas Infections
Viability assay
Drug Carriers
Mice, Inbred BALB C
Pseudomonas aeruginosa
business.industry
021001 nanoscience & nanotechnology
medicine.disease
Lipids
Nanostructures
Female
Nanocarriers
0210 nano-technology
Drug carrier
business
medicine.drug
Subjects
Details
- ISSN :
- 03785173
- Volume :
- 498
- Database :
- OpenAIRE
- Journal :
- International Journal of Pharmaceutics
- Accession number :
- edsair.doi.dedup.....6e751debcc654417d5102465438cb487