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Reactive Oxygen Species Participate in the Control of Mouse Embryonic Cell Death
- Source :
- Experimental Cell Research. 238:136-147
- Publication Year :
- 1998
- Publisher :
- Elsevier BV, 1998.
-
Abstract
- Programmed cell death or apoptosis is an essential process during the morphogenesis of a large number of structures. Evidence obtained over the past few years indicates that, in some cases, the generation of reactive oxygen species (ROS) is an important event during the course of apoptosis. Using an in vitro culture system in which digit individualization of developing limbs normally occurs, we assayed the effect of different antioxidants on the cell death that takes place at interdigits. The addition of phenol, dimethyl sulfoxide, or 2',7'-dichlorodihydrofluorescein diacetate (DCDHF-DA) to murine developing limbs in culture prevented digit individualization as well as the typical interdigital cell death. Two ROS-sensitive dyes, 3-(4,5-dimethylthiazol)-2,5-diphenyl tetrazolium bromide and DCDHF-DA, stained interdigits and the so-called "necrotic zones," implying that they contain cells under oxidative stress. Very few interdigital cells were doubly stained with the ROS probes and two cell death indicators (i.e., acridine orange and propidium iodide), suggesting that they detect a different stage during the course of apoptosis. Furthermore, we found cells stained for ROS that did not express a specific macrophage marker and in a few cases were seen surrounded by a macrophage. Surprisingly, many regions of the midgestation mouse embryo that are undergoing cell death correlated with those that have a markedly higher level of ROS. Our data suggest that the generation of oxidative stress is a common requirement for cell death that occurs during mouse embryonic development.
- Subjects :
- Programmed cell death
Limb Buds
Morphogenesis
Apoptosis
Biology
medicine.disease_cause
Antioxidants
Embryonic and Fetal Development
Mice
chemistry.chemical_compound
Organ Culture Techniques
medicine
Animals
Dimethyl Sulfoxide
Propidium iodide
Fluorescent Dyes
chemistry.chemical_classification
Reactive oxygen species
Microscopy, Confocal
Phenol
Macrophages
Acridine orange
Cell Biology
Embryo, Mammalian
Fluoresceins
Embryonic stem cell
Molecular biology
chemistry
Reactive Oxygen Species
Oxidative stress
Subjects
Details
- ISSN :
- 00144827
- Volume :
- 238
- Database :
- OpenAIRE
- Journal :
- Experimental Cell Research
- Accession number :
- edsair.doi.dedup.....6e74a38afa67b6e66eacdd8a2c5085c6