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Contribution of neutrophil-derived myeloperoxidase in the early phase of fulminant acute respiratory distress syndrome induced by influenza virus infection
- Source :
- Microbiology and Immunology. 56:171-182
- Publication Year :
- 2012
- Publisher :
- Wiley, 2012.
-
Abstract
- Because the pathogenesis of acute respiratory distress syndrome (ARDS) induced by influenza virus infection remains unknown, we can only improve on existing therapeutic interventions. To approach the subject, we investigated immunological etiology focused on cytokines and an acute lung damage factor in influenza-induced ARDS by using a PR-8 (A/H1N1)-infected mouse model. The infected mouse showed fulminant severe pneumonia with leukocyte infiltration, claudin alteration on tight junctions, and formation of hyaline membranes. In addition to interferon (IFN)-α, plenty of keratinocyte-derived chemokines (KC), macrophage inflammatory protein 2 (MIP-2), regulated on activation normal T-cell expressed and secreted (RANTES), and monocyte chemotactic protein 1 (MCP-1) were significantly released into bronchoalveolar lavage fluid (BALF) of the model. We focused on neutrophil myeloperoxidase (MPO) as a potent tissue damage factor and examined its contribution in influenza pneumonia by using mice genetically lacking in MPO. The absence of MPO reduced inflammatory damage with suppression of leakage of total BALF proteins associated with alteration of claudins in the lung. MPO(-/-) mice also suppressed viral load in the lung. The present study suggests that MPO-mediated OCl(-) generation affects claudin molecules and leads to protein leakage and viral spread as a damage factor in influenza-induced ARDS.
- Subjects :
- Chemokine
ARDS
Neutrophils
Fulminant
Pneumonia, Viral
Immunology
medicine.disease_cause
Microbiology
Pathogenesis
Mice
Influenza A Virus, H1N1 Subtype
Orthomyxoviridae Infections
Virology
medicine
Influenza A virus
Animals
Claudin
Peroxidase
Mice, Knockout
Mice, Inbred BALB C
Respiratory Distress Syndrome
medicine.diagnostic_test
biology
medicine.disease
respiratory tract diseases
Bronchoalveolar lavage
Myeloperoxidase
biology.protein
Cytokines
Female
Subjects
Details
- ISSN :
- 03855600
- Volume :
- 56
- Database :
- OpenAIRE
- Journal :
- Microbiology and Immunology
- Accession number :
- edsair.doi.dedup.....6e1902186dc47307e84eea76a6b7e6e1