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In Vivo Imaging of Experimental Melanoma Tumors using the Novel Radiotracer 68Ga-NODAGA-Procainamide (PCA)
- Source :
- Journal of Cancer
- Publication Year :
- 2017
- Publisher :
- Ivyspring International Publisher, 2017.
-
Abstract
- Purpose: The most aggressive form of skin cancer is the malignant melanoma. Because of its high metastatic potential the early detection of primary melanoma tumors and metastases using non-invasive PET imaging determines the outcome of the disease. Previous studies have already shown that benzamide derivatives, such as procainamide (PCA) specifically bind to melanin pigment. The aim of this study was to synthesize and investigate the melanin specificity of the novel 68Ga-labeled NODAGA-PCA molecule in vitro and in vivo using PET techniques. Methods: Procainamide (PCA) was conjugated with NODAGA chelator and was labeled with Ga-68 (68Ga-NODAGA-PCA). The melanin specificity of 68Ga-NODAGA-PCA was tested in vitro, ex vivo and in vivo using melanotic B16-F10 and amelanotic Melur melanoma cell lines. By subcutaneous and intravenous injection of melanoma cells tumor-bearing mice were prepared, on which biodistribution studies and small animal PET/CT scans were performed for 68Ga-NODAGA-PCA and 18FDG tracers. Results: 68Ga-NODAGA-PCA was produced with high specific activity (14.9±3.9 GBq/µmol) and with excellent radiochemical purity (98%
- Subjects :
- Biodistribution
Pathology
medicine.medical_specialty
Klinikai orvostudományok
030218 nuclear medicine & medical imaging
Melanin
03 medical and health sciences
0302 clinical medicine
In vivo
Positron Emission Tomography, Procainamide
medicine
18FDG
Chemistry
Melanoma
Melanoma malignum
Orvostudományok
68Ga-NODAGA-PCA
medicine.disease
In vitro
Oncology
030220 oncology & carcinogenesis
B16-F10 tumor
Skin cancer
Ex vivo
Preclinical imaging
Research Paper
Subjects
Details
- Language :
- English
- ISSN :
- 18379664
- Volume :
- 8
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Journal of Cancer
- Accession number :
- edsair.doi.dedup.....6e10f25dfd9e70774a37d5d539daa55d