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The requirement of yeast replication origins for pre-replication complex proteins is modulated by transcription
- Source :
- Nucleic Acids Research
- Publication Year :
- 2005
- Publisher :
- Oxford University Press, 2005.
-
Abstract
- The mini-chromosome maintenance proteins Mcm2–7 are essential for DNA replication. They are loaded onto replication origins during G1 phase of the cell cycle to form a pre-replication complex (pre-RC) that licenses each origin for subsequent initiation. We have investigated the DNA elements that determine the dependence of yeast replication origins on Mcm2–7 activity, i.e. the sensitivity of an origin to mcm mutations. Using chimaeric constructs from mcm sensitive and mcm insensitive origins, we have identified two main elements affecting the requirement for Mcm2–7 function. First, transcription into an origin increases its dependence on Mcm2–7 function, revealing a conflict between pre-RC assembly and transcription. Second, sequence elements within the minimal origin influence its mcm sensitivity. Replication origins show similar differences in sensitivity to mutations in other pre-RC proteins (such as Origin Recognition Complex and Cdc6), but not to mutations in initiation and elongation factors, demonstrating that the mcm sensitivity of an origin is determined by its ability to establish a pre-RC. We propose that there is a hierarchy of replication origins with respect to the range of pre-RC protein concentrations under which they will function. This hierarchy is both ‘hard-wired’ by the minimal origin sequences and ‘soft-wired’ by local transcriptional context.
- Subjects :
- DNA Replication
Saccharomyces cerevisiae Proteins
Transcription, Genetic
Eukaryotic DNA replication
Cell Cycle Proteins
Replication Origin
Saccharomyces cerevisiae
Pre-replication complex
Article
Chromosomes
DNA replication factor CDT1
Evolution, Molecular
03 medical and health sciences
0302 clinical medicine
Replication factor C
Minichromosome maintenance
Control of chromosome duplication
Genetics
DNA, Fungal
030304 developmental biology
0303 health sciences
Binding Sites
biology
Nuclear Proteins
Sequence Analysis, DNA
DNA-Binding Proteins
Licensing factor
Phenotype
Mutation
biology.protein
Origin recognition complex
030217 neurology & neurosurgery
Plasmids
Subjects
Details
- Language :
- English
- ISSN :
- 13624962 and 03051048
- Volume :
- 33
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Research
- Accession number :
- edsair.doi.dedup.....6e061dd031cce5a48bac9989fa8e20f1