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Immunohistochemical localization and pharmacokinetics of the anti-MRSA drug teicoplanin in rat kidney using a newly developed specific antibody
- Source :
- Medical Molecular Morphology. 54:227-236
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- We prepared a polyclonal antibody against a teicoplanin (TEIC)-bovine serum albumin conjugate that was specific to both conjugated and free forms of TEIC. We demonstrated that this antibody could be used to detect the time-dependent localization of TEIC in rat kidneys. Immunohistochemistry revealed immunoreactivity specifically in the microvilli and apical cytoplasm of epithelial cells in proximal tubule segments S1 and S2, 1 h after intravenous TEIC injection, with higher staining intensity in the S2 segments. The epithelial cells of S3 segments showed moderate immunostaining with a few cells exhibiting nuclear staining. Furthermore, we found that the distal tubules and collecting ducts contained both TEIC-positive and -negative cells. TEIC immunoreactivity decreased rapidly over time; only weak staining remained in the S3 segments, distal tubules, and collecting ducts 24 h after administration. No staining was detected 7 days after injection. These results were significantly different from those of our previous study obtained using vancomycin, which showed moderate staining in the proximal tubule segments S1 and S2, distal tubules, and the collecting ducts 8 days after administration. The lower TEIC accumulation in tissues may account for a lower risk of adverse events compared to that using vancomycin.
- Subjects :
- 0301 basic medicine
Serum albumin
Kidney
Antibodies
Pathology and Forensic Medicine
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Apical cytoplasm
Molecular Biology
biology
Chemistry
General Medicine
Immunohistochemistry
Molecular biology
Anti-Bacterial Agents
Rats
Staining
030104 developmental biology
medicine.anatomical_structure
Polyclonal antibodies
030220 oncology & carcinogenesis
Injections, Intravenous
biology.protein
Teicoplanin
Antibody
Immunostaining
Subjects
Details
- ISSN :
- 18601499 and 18601480
- Volume :
- 54
- Database :
- OpenAIRE
- Journal :
- Medical Molecular Morphology
- Accession number :
- edsair.doi.dedup.....6e0221870cfa8292ce266d57d150a6c1