Back to Search
Start Over
Identification of a novel receptor-like protein kinase that interacts with a geminivirus nuclear shuttle protein
- Source :
- LOCUS Repositório Institucional da UFV, Universidade Federal de Viçosa (UFV), instacron:UFV
- Publication Year :
- 2004
- Publisher :
- Elsevier BV, 2004.
-
Abstract
- Despite extensive studies in plant virus–host interactions, the molecular mechanisms of geminivirus movement and interactions with host components remain largely unknown. A tomato kinase protein and its soybean homolog were found to interact specifically with the nuclear shuttle protein (NSP) of Tomato golden mosaic virus (TGMV) and Tomato crinkle leaf yellows virus (TCrLYV) through yeast two-hybrid screening and in vitro protein binding assays. These proteins, designated LeNIK (Lycopersicon esculentum NSP-Interacting Kinase) and GmNIK (Glycine max NIK), belong to the LRR-RLK (leucine rich-repeat receptor-like kinase) family that is involved in plant developmental processes and/or resistance response. As such, NIK is structurally organized into characteristic domains, including a serine/threonine kinase domain with a nucleotide binding site at the C-terminal region, an internal transmembrane segment and leucine-rich repeats (LRR) at the N-terminal portion. The potential significance of the NSP–NIK interaction is discussed.
- Subjects :
- Molecular Sequence Data
Nuclear shuttle protein
Protein Serine-Threonine Kinases
Biology
MAP3K7
Tomato kinase protein
MAP2K7
Viral Proteins
Solanum lycopersicum
Two-Hybrid System Techniques
Virology
Amino Acid Sequence
c-Raf
Binding site
Nuclear protein
Protein kinase A
Plant Proteins
fungi
Nuclear Proteins
food and beverages
Plant Viral Movement Proteins
Transmembrane domain
Geminiviridae
Biochemistry
Protein kinase domain
Geminivirus
Subjects
Details
- ISSN :
- 00426822
- Volume :
- 318
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....6dfc8806de2df5a406f875167d95d7cc
- Full Text :
- https://doi.org/10.1016/j.virol.2003.09.038