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Identification of a novel receptor-like protein kinase that interacts with a geminivirus nuclear shuttle protein

Authors :
Maxuel O. Andrade
Maria Cristina Baracat-Pereira
Andrea C. Mariano
Sônia M.B. Carolino
Elizabeth P. B. Fontes
Sérgio H. Brommonshenkel
Marli L. Oliveira
Anésia A. Santos
Source :
LOCUS Repositório Institucional da UFV, Universidade Federal de Viçosa (UFV), instacron:UFV
Publication Year :
2004
Publisher :
Elsevier BV, 2004.

Abstract

Despite extensive studies in plant virus–host interactions, the molecular mechanisms of geminivirus movement and interactions with host components remain largely unknown. A tomato kinase protein and its soybean homolog were found to interact specifically with the nuclear shuttle protein (NSP) of Tomato golden mosaic virus (TGMV) and Tomato crinkle leaf yellows virus (TCrLYV) through yeast two-hybrid screening and in vitro protein binding assays. These proteins, designated LeNIK (Lycopersicon esculentum NSP-Interacting Kinase) and GmNIK (Glycine max NIK), belong to the LRR-RLK (leucine rich-repeat receptor-like kinase) family that is involved in plant developmental processes and/or resistance response. As such, NIK is structurally organized into characteristic domains, including a serine/threonine kinase domain with a nucleotide binding site at the C-terminal region, an internal transmembrane segment and leucine-rich repeats (LRR) at the N-terminal portion. The potential significance of the NSP–NIK interaction is discussed.

Details

ISSN :
00426822
Volume :
318
Issue :
1
Database :
OpenAIRE
Journal :
Virology
Accession number :
edsair.doi.dedup.....6dfc8806de2df5a406f875167d95d7cc
Full Text :
https://doi.org/10.1016/j.virol.2003.09.038