The Effect of Yeast and Roughage Concentrate Ratio on Ruminal pH and Protozoal Population in Thai Native Beef Cattle

Simple Summary As a result of the recent ban on antibiotics in feed, animal probiotics are becoming increasingly popular. Yeast is extensively used as both a probiotic and prebiotic in the gastrointestinal tracts of ruminants. The purpose of this study is to determine how adding yeast (Saccharomyces cerevisiae) to the diet and changing the roughage-to-concentrate ratio (R:C ratio) affects nutrient consumption, rumen fermentation, microbial protein synthesis, and protozoal population in Thai native beef cattle. The roughage source was urea–calcium-hydroxide-treated rice straw. The findings suggest that supplementing with a R:C ratio of 40:60 and a LY of 4 g/hd/d boosted nutrient digestibility, volatile fatty acid (VFA) production, propionic acid (C3) in particular, and microbial protein synthesis while lowering protozoal population. Abstract The objective of this research is to investigate the effect of yeast (Saccharomyces cerevisiae) adding and roughage-to-concentrate ratio (R:C ratio) on nutrients utilization, rumen fermentation efficiency, microbial protein synthesis, and protozoal population in Thai native beef cattle. Four Thai native beef cattle, weighing an average of 120 ± 10 kg live weight, were randomly assigned to four dietary treatments using a 2 × 2 factorial arrangement in a 4 × 4 Latin square design. Factor A was the level of roughage-to-concentrate ratio (R:C ratio) at 60:40 and 40:60; factor B was the levels of live yeast (LY) supplementation at 0 and 4 g/hd/d; urea–calcium-hydroxide-treated rice straw were used as a roughage source. Findings revealed that total intake and digestibility of dry matter (DM), organic matter (OM), and crude protein (CP) were increased (p < 0.05) by both factors, being greater for steers fed a R:C ratio of 40:60 supplemented with 4 g LY/hd/d. Ruminal ammonia nitrogen, total volatile fatty acid (VFA), and propionate (C3) were increased (p < 0.05) at the R:C ratio of 40:60 with LY supplementation at 4 g/hd/d, whereas rumen acetate (C2) and the C2 to C3 ratio were decreased (p < 0.05). With a high level of concentrate, LY addition increased total bacterial direct counts and fungal zoospores (p < 0.05), but decreased protozoal populations (p < 0.05). High-concentrate diet and LY supplementation increased nitrogen absorption and the efficiency of microbial nitrogen protein production. In conclusion, feeding beef cattle with 4 g/hd/d LY at a R:C ratio of 40:60 increased C3 and nutritional digestibility while lowering protozoal population.