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Developmental patterns in the antioxidant defenses of the housefly, Musca domestica
- Source :
- Journal of cellular physiology. 146(2)
- Publication Year :
- 1991
-
Abstract
- The activities of superoxide dismutase (SOD), catalase, glutathione peroxidase, glutathione S-transferases, GSSG reductase, thiol transferases, gamma glutamyl-cysteine synthetase, and glucose-6-phosphate dehydrogenase, and the concentrations of H2O2 and reduced and oxidized glutathione were determined in the various developmental stages of houseflies. Housefly development was correlated with a progressive increase of cellular oxidizing equivalents and a loss of cellular reducing capacity. The loss of reducing equivalents appeared to result from a decrease in the activity of enzymes involved in glutathione and NADPH synthesis and a concomitant increase in glutathione-oxidizing enzymes. Relatively little change was observed in SOD activity during housefly development; however, the electrophoretic pattern of MnSOD varied in a manner specific to developmental stage. A striking increase in H2O2 concentration occurred prior to pupation possibly due to changes in substrate catabolism. These results support the hypothesis that the cellular environment becomes progressively more oxidizing during development.
- Subjects :
- Antioxidant
Physiology
medicine.medical_treatment
Clinical Biochemistry
Glutathione reductase
Superoxide dismutase
chemistry.chemical_compound
Houseflies
medicine
Animals
Housefly
chemistry.chemical_classification
biology
Catabolism
Superoxide Dismutase
Glutathione peroxidase
Pupa
Cell Biology
Glutathione
Hydrogen Peroxide
biology.organism_classification
Catalase
Glutathione Reductase
Biochemistry
chemistry
Larva
biology.protein
Oxidoreductases
Subjects
Details
- ISSN :
- 00219541
- Volume :
- 146
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of cellular physiology
- Accession number :
- edsair.doi.dedup.....6dd28eb788d6a568cb8d432ac694885b