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Secretome signature of cardiopoietic cells echoed in rescued infarcted heart proteome
- Source :
- Stem Cells Translational Medicine, Vol 10, Iss 9, Pp 1320-1328 (2021), Stem Cells Translational Medicine
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Stem cell paracrine activity is implicated in cardiac repair. Linkage between secretome functionality and therapeutic outcome was here interrogated by systems analytics of biobanked human cardiopoietic cells, a regenerative biologic in advanced clinical trials. Protein chip array identified 155 proteins differentially secreted by cardiopoietic cells with clinical benefit, expanded into a 520 node network, collectively revealing inherent vasculogenic properties along with cardiac and smooth muscle differentiation and development. Next generation RNA sequencing, refined by pathway analysis, pinpointed miR‐146 dependent regulation upstream of the decoded secretome. Intracellular and extracellular integration unmasked commonality across cardio‐vasculogenic processes. Mirroring the secretome pattern, infarcted hearts benefiting from cardiopoietic cell therapy restored the disease proteome engaging cardiovascular system functions. The cardiopoietic cell secretome thus confers a therapeutic molecular imprint on recipient hearts, with response informed by predictive systems profiling.<br />Reverse translational decoding characterized the secretome of cardiopoietic cells, a regenerative therapeutic in clinical testing. A cardiovasculogenic systems signature distinguished high from low response profiles, an imprint echoed in the restored diseased proteome. Linkage of realized outcome with secretome identity suggests paracrine centrality in regenerative fitness. Pre‐intervention secretome profiling would thus inform optimized selection of regenerative biologic candidates.
- Subjects :
- 0301 basic medicine
Medicine (General)
Proteome
Clinomics
Systems biology
Cell
Myocardial Infarction
heart failure
regenerative medicine
Biology
Regenerative medicine
Cell therapy
03 medical and health sciences
0302 clinical medicine
R5-920
cardiopoiesis
stem cells
medicine
Humans
clinomics
Secretome
therapy
QH573-671
Heart
systems biology
Cell Biology
General Medicine
Cell biology
030104 developmental biology
medicine.anatomical_structure
Mesenchymal Stem Cells
Enabling Technologies for Cell‐based Clinical Translation
Stem cell
Cytology
030217 neurology & neurosurgery
Intracellular
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 21576564 and 21576580
- Volume :
- 10
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Stem Cells Translational Medicine
- Accession number :
- edsair.doi.dedup.....6dc93634ed0c20efce4ba94ce509eb90