Back to Search
Start Over
Pre-operative intracellular glutathione levels of peripheral monocytes as a biomarker to predict survival of colorectal cancer patients
- Source :
- Cancer Immunology, Immunotherapy. 59:1457-1465
- Publication Year :
- 2010
- Publisher :
- Springer Science and Business Media LLC, 2010.
-
Abstract
- The ability to predict anti-tumor immune responses at local tumor growing sites using only peripheral blood specimens would be helpful in determining therapeutic options for patients with solid tumors. Here, we show that the glutathione intracellular content (icGSH) of peripheral monocytes (Mo) correlates positively with T cell infiltration within tumor islets and overall survival in patients with colorectal carcinoma. IcGSH redox status was determined in CD14(+) Mo prior to surgery by staining with monochlorobimane. The tumor-infiltrating T cells (TIL) were quantified as CD45RO(+) T cells in resected tumors using paraffin sections. A positive association was found between the GSH index and TIL in tumor islets (P0.001). The 50% cut-off value for the GSH index, that is the determinant between TIL presence or absence in tumor islets, was calculated to be almost 0.7 through logistic regression analysis. Mo with a GSH index ofor =0.7 were termed reductive (R)-Mo, and those with0.7 were designated as oxidative (O)-Mo. Cox's proportional hazards regression analysis of patients with R-Mo or O-Mo prior to surgery, and the presence or absence of TIL, was found to correlate significantly with the overall survival rate of stage II and III patients. Kaplan-Meier analysis also showed a significant correlation. These results indicate that the Mo icGSH index is a useful biomarker parameter for better understanding the host/tumor relationship prior to surgery, thereby enabling the development of an individual patient-oriented therapeutic strategy.
- Subjects :
- Male
Cancer Research
Pathology
medicine.medical_specialty
Colorectal cancer
medicine.medical_treatment
CD14
Immunology
Immune system
Predictive Value of Tests
Preoperative Care
Biomarkers, Tumor
medicine
Humans
Immunology and Allergy
Chromatography, High Pressure Liquid
Survival analysis
Neoplasm Staging
business.industry
Monocyte
Cancer
Immunotherapy
Middle Aged
Reference Standards
Prognosis
medicine.disease
Glutathione
Survival Analysis
Treatment Outcome
medicine.anatomical_structure
Oncology
Leukocytes, Mononuclear
Cancer research
Biomarker (medicine)
Female
Colorectal Neoplasms
business
Oxidation-Reduction
Subjects
Details
- ISSN :
- 14320851 and 03407004
- Volume :
- 59
- Database :
- OpenAIRE
- Journal :
- Cancer Immunology, Immunotherapy
- Accession number :
- edsair.doi.dedup.....6db73d11efcc99295a833d82defef98d