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Sulfated modification of the polysaccharide from Sphallerocarpus gracilis and its antioxidant activities
- Source :
- International Journal of Biological Macromolecules. 87:180-190
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Sphallerocarpus gracilis (S. gracilis) is a little-investigated edible plant and used as traditional Chinese medicine. In this study, polysaccharide extracted from S. gracilis,deproteined and purified. The polysaccharide (SGP) was chemically modified to obtain its sulfated derivatives (S-SGP) using the method of chlorosulfonic acid/pyridine (CSA/Pyr). In order to acquire the derivative with the highest degree of substitution (DS), the optimum conditions of the sulfation were obtained based on response surface design (RSD), and the structural characterizations and antioxidant properties of the S-SGP were comparatively investigated by Fourier transform infrared spectrometry (FT-IR), GC-MS analysis, size exclusion chromatography (SEC), and DPPH radical assay, hydroxyl radical assay, superoxide radical assay, and reducing power assay, respectively. Results showed that the modification was successful, and obtained the optimum combination of conditions. Compared with SGP, the sulfated polysaccharide with relatively the decreased degree of molecular weight (Mw) but the same composition of monosaccharides exhibited better antioxidant activities in DPPH, hydroxyl, superoxide radical and reducing power assay. These results indicated that the antioxidant activities in vitro of the S-SGP from S. gracilis may be related to combined effects of Mw, monosaccharide composition, and sulfate content.
- Subjects :
- Antioxidant
Free Radicals
DPPH
medicine.medical_treatment
Chemical structure
Size-exclusion chromatography
02 engineering and technology
Polysaccharide
01 natural sciences
Biochemistry
Antioxidants
chemistry.chemical_compound
Sulfation
Polysaccharides
Structural Biology
medicine
Monosaccharide
Molecular Biology
chemistry.chemical_classification
Chromatography
Sulfates
010405 organic chemistry
Monosaccharides
General Medicine
021001 nanoscience & nanotechnology
0104 chemical sciences
Molecular Weight
chemistry
Regression Analysis
Hydroxyl radical
0210 nano-technology
Oxidation-Reduction
Apiaceae
Subjects
Details
- ISSN :
- 01418130
- Volume :
- 87
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Macromolecules
- Accession number :
- edsair.doi.dedup.....6d95a074873fc210774916abe550c096
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2016.02.037