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Biogenesis of a mitochondrial DNA inheritance machinery in the mitochondrial outer membrane
- Publication Year :
- 2017
- Publisher :
- Cold Spring Harbor Laboratory, 2017.
-
Abstract
- Mitochondria cannot form de novo but require mechanisms that mediate their inheritance to daughter cells. The parasitic protozoanTrypanosoma bruceihas a single mitochondrion with a single-unit genome that is physically connected across the mitochondrial membranes to the basal body of the flagellum. This connection, termed tripartite attachment complex (TAC), is essential for the segregation of the replicated mitochondrial genomes prior to cytokinesis. Here we identify a protein complex consisting of three integral mitochondrial outer membrane proteins - TAC60, TAC42 and TAC40 - which are essential subunits of the TAC. TAC60 contains separable mitochondrial import and TAC-sorting signals and its biogenesis depends on the main outer membrane protein translocase. TAC40 is a member of the mitochondrial porin family, whereas TAC42 represents a novel class of mitochondrial outer membrane β-barrel proteins. Consequently TAC40 and TAC42 contain C-terminal β-signals. Thus in trypanosomes the highly conserved β-barrel protein assembly machinery plays a major role in the biogenesis of its unique mitochondrial genome segregation system.
- Subjects :
- 0303 health sciences
Mitochondrial DNA inheritance
biology
Translocase of the outer membrane
030302 biochemistry & molecular biology
TIM/TOM complex
Mitochondrial carrier
Cell biology
03 medical and health sciences
Mitochondrial membrane transport protein
mitochondrial fusion
Translocase of the inner membrane
biology.protein
ATP–ADP translocase
030304 developmental biology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....6d933576284d024664321698c92699cc
- Full Text :
- https://doi.org/10.1101/190751