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Real-time imaging of mechanically injured femoral artery in mice reveals a biphasic pattern of leukocyte accumulation
- Source :
- American Journal of Physiology-Heart and Circulatory Physiology. 292:H1876-H1882
- Publication Year :
- 2007
- Publisher :
- American Physiological Society, 2007.
-
Abstract
- Wire injury of an artery has been recognized as a standard model of vascular inflammation and atherosclerosis; however, the mechanism of leukocyte recruitment has not been studied in this model. In this study, we documented the recruitment of leukocytes to the murine femoral artery after a wire injury. A transluminal mechanical injury was generated by insertion of a wire into the femoral artery of male C57BL/6J mice. The mice were anesthetized and ventilated after tracheotomy and protected from hypothermia by a warming lamp. Body temperature and blood pH did not significantly change during the experiment. The interaction between rhodamine 6G-labeled leukocytes and the injured femoral artery was monitored using an epifluorescent microscope, and the images were evaluated using a computer-assisted image analysis program. In the absence of injury, virtually no leukocyte adhesion was observed. In contrast, the number of adherent leukocytes increased 4 and 24 h after injury and declined 72 h after injury. The rolling flux of leukocytes increased 4 h after injury and remained high up to 7 days, but it was faster 72 h after injury. We identified another peak of leukocyte adhesion 7 days after injury. Injection of anti-P-selectin antibody significantly reduced leukocyte adhesion at the early and later phases. In conclusion, we have established a novel experimental system for direct observation of leukocyte recruitment to the injured femoral artery. Our system revealed a previously undetected, unique profile of leukocyte recruitment during vascular injury.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Physiology
Adhesion (medicine)
Inflammation
Femoral artery
Lesion
Leukocyte Count
Mice
Cell Movement
Computer Systems
Physiology (medical)
medicine.artery
Leukocytes
medicine
Animals
Arteritis
Microscopy, Video
Interleukin-6
business.industry
Hypothermia
medicine.disease
Femoral Artery
Mice, Inbred C57BL
P-Selectin
medicine.anatomical_structure
Circulatory system
medicine.symptom
Cardiology and Cardiovascular Medicine
business
Biomarkers
Intravital microscopy
Artery
Subjects
Details
- ISSN :
- 15221539 and 03636135
- Volume :
- 292
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Heart and Circulatory Physiology
- Accession number :
- edsair.doi.dedup.....6d930199367d851e67c6885d96ee3a5a